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Cat. No.: HY-187208

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METTL3-IN-14

METTL3-IN-14 is a METTL3 inhibitor with a target IC₅₀ of 6.68 μM and target K_d of 8.7 μM. METTL3-IN-14 binds to the METTL3 active site, inhibits methyltransferase activity, and does not alter METTL3 mRNA or protein expression levels. METTL3-IN-14 acts as an antiviral agent, exerts anti-SARS-CoV-2 activity via non-METTL3 targets. METTL3-IN-14 can be used for the research of covid-19.

For research use only. We do not sell to patients.

Formula: C₃₃H₃₄N₄O₂
Molecular Weight:518.65

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

METTL3-IN-14 Chemical Structure

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

In Vitro

METTL3-IN-14 (Compound D4) (0.3-3 μM; 1-48 h) potently inhibits SARS-CoV-2 replication in Vero E6 cells, with an EC₅₀ of 0.31 μM, a CC₅₀ > 200 μM, and a selectivity index exceeding 645^[1].
METTL3-IN-14 (48 h) exhibits broad-spectrum antiviral activity against SARS-CoV-2 variants XBB.1.9.1, BA.5.2.48 and BA.2 in Vero E6 cells, with EC₅₀ values of 0.26 μM, 0.72 μM and 0.56 μM, respectively^[1].
METTL3-IN-14 (0-200 μM; 72 h) exhibits low cytotoxicity in 293T, Caco-2, Calu-3, HaCaT and BEAS-2B cells, with a CC₅₀ value of > 200 μM in most cell lines and 145 μM in 293T cells^[1].
METTL3-IN-14 (0.3-6 μM; 48 h) exerts no significant effect on the mRNA or protein expression levels of METTL3 in Calu-3 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:

293T, Caco-2, Calu-3, HaCaT, BEAS-2B
Concentration:

0, 6.25, 12.5, 25, 50, 100 and 200 μM
Incubation Time:

72 h
Result:

Had a CC₅₀ > 200 μM in HaCaT, BEAS-2B, Caco-2, and Calu-3 cells.
Had a CC₅₀ of 145 μM in 293T cells.
Showed consistently higher CC₅₀ values compared to vilazodone and remdesivir across all tested cell lines.

Chemical Information

Molecular Weight 518.65
Formula C₃₃H₃₄N₄O₂
SMILES

OCC(O1)=CC2=C1C=CC(N(CC3)CCN3CCCCC4=CN(CC5=CC=CC=C5)C6=CC=C(C#N)C=C64)=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Sun XL, et al. Design, synthesis, and anti-SARS-CoV-2 activity of vilazodone derivatives as METTL3 inhibitors. European journal of medicinal chemistry. 2026 Jun 29;317:119114.

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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