N-TCO-L-lysine
Based on 1 Customer Validation
N-TCO-L-lysine is a non-canonical amino acid. N-TCO-L-lysine contains a trans-cyclooctene (TCO) bioorthogonal reactive linker. N-TCO-L-lysine undergoes a bioorthogonal click reaction with SiR-Tz to enable fluorescent labeling of endogenously expressed proteins with site-specific incorporation. When used in combination with SiR-Tz, N-TCO-L-lysine allows super-resolution and live-cell imaging of endogenous proteins.
For research use only. We do not sell to patients.
- Purity: 99.06%
- CAS No.: 1380349-88-1
- Formula: C15H26N2O4
- Molecular Weight:298.38
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guidelines (The following is our recommended protocol, which serves only as a guideline and should be modified according to your specific needs).
1.1 Preparation of Stock Solution[1]
Prepare a 250 mM N-TCO-L-lysine solution as the stock solution.
1.2 Preparation of Working Solution
Dilute the 250 mM N-TCO-L-lysine solution 1000-fold with McCoy’s 5a medium.
1.3 Experimental Procedures
1) Add the N-TCO-L-lysine working solution to cells to reach a final concentration of 250 μM.
2) Culture the cells at 37 °C under 5% CO2 for 48 hours, followed by washing with fresh medium. To remove excess N-TCO-L-lysine, wash the cells three times with fresh medium (1 hour each time).
3) Then incubate the cells with a tetrazine dye (5 μM) diluted in fresh medium at 37 °C under 5% CO2 for 10 minutes.
4) After labeling, wash the cells twice with fresh medium, then continue culturing them at 37 °C under 5% CO2 for 2-3 hours.
5) Image the resulting live cells using a confocal laser scanning microscope.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1380349-88-1
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Appearance Solid
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Molecular Weight 298.38
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Formula C15H26N2O4
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Color White to off-white
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SMILES
OC([C@@H](N)CCCCNC(OC1CCC/C=C\CC1)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 4 mg/mL (13.41 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (269 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.3514 mL | 16.7572 mL | 33.5143 mL | 83.7858 mL |
| 5 mM | 0.6703 mL | 3.3514 mL | 6.7029 mL | 16.7572 mL | |
| 10 mM | 0.3351 mL | 1.6757 mL | 3.3514 mL | 8.3786 mL |
N-TCO-L-lysine Related Classifications
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