Protease Substrate
- Formula: C24H35NO18
- Molecular Weight: 625.53
4-Nitrophenyl β-D-cellotrioside (p-Nitopheyl β-D-cellotioside) is a chromogenic substrate for endoglucanases and cellulose biohydrolases. As a fluorescent dye, nitrophenyl β-D-Cellotrioside can be hydrolyzed by enzymes to release 4-nitrophenol, producing a yellow color. The activity of the enzyme can be quantitatively analyzed by monitoring the change in absorbance at 405 nm.
- Formula: C19H22N2O9
- Molecular Weight: 422.39
AMC-GlcNAc (compound 1) is a potent fluorogenic probe for detection and characterization of β-hexosaminidase enzyme activity. AMC-GlcNAc enables continuous monitoring by turn-on fluorescence and fluorescence signal is constant over a wide pH range (Ex=325 nm, Em=390 nm). AMC-GlcNAc enables ratiometric fluorescence detection with high sensitivity and low background, which can be used to screen for recombinant dispersin B activity in E. coli cell lysate.
- Formula: C31H43N9O7
- Molecular Weight: 653.73
D-Lys(Z)-Pro-Arg-pNA (Chromozym Pca) is a chromogenic peptide substrate of activated protein C (APC).
