Phosphoglycerate kinase, yeast  (Synonyms: PGK)

Phosphoglycerate kinase (PGK), yeast is a monomeric glycolytic/gluconeogenic enzyme that can be found in Saccharomyces cerevisiae. Phosphoglycerate kinase, yeast catalyzes phosphoryl-group transfer from 1,3-bisphosphoglycerate to ADP to form 3-phosphoglycerate and ATP, binds 3PGA, ATP, and Mg²⁺, and adopts a closed conformation. Phosphoglycerate kinase, yeast is commonly used in biochemical research^[1][2][3].

Phosphoglycerate kinase, yeast is a monomeric enzyme that forms a closed conformation complex with 3PGA, ATP, and Mg^2+[1].
Phosphoglycerate kinase, yeast can utilize GTP and ITP as substrates^[1].
Phosphoglycerate kinase, yeast does not adopt a closed catalytic conformation when complexed with 3-PGA and MgAMP-PNP^[2].
Phosphoglycerate kinase, yeast (5 μg/mL; 30 min) shows negligible plasmin reductase activity in an ELISA assay using purified plasmin as substrate^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9001-83-6

Viscous Liquid

Colorless to light yellow

[Phosphoglycerate kinase, yeast]

2.7.2.3

≥1000 U/mg protein

One unit will convert 1.0 μmole of 1,3-diphosphoglycerate to 3-phosphoglycerate per min at pH 6.9 at 25 °C.

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Rojas-Pirela M, et al. Phosphoglycerate kinase: structural aspects and functions, with special emphasis on the enzyme from Kinetoplastea. Open Biol. 2020 Nov;10(11):200302.  [Content Brief]

  [2]. Bernstein BE, Michels PA, Hol WG. Synergistic effects of substrate-induced conformational changes in phosphoglycerate kinase activation. Nature. 1997 Jan 16;385(6613):275-8.  [Content Brief]

  [3]. Lay AJ, Jiang XM, Kisker O, et al.. Phosphoglycerate kinase acts in tumour angiogenesis as a disulphide reductase. Nature. 2000 Dec 14;408(6814):869-73.  [Content Brief]