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Poly(ethylene glycol) diacrylate (MW 10000)  (Synonyms: PEGDA (MW 10000))

Cat. No.: HY-W110542H Purity: 98.0%
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Handling Instructions Technical Support

Poly(ethylene glycol) diacrylate (PEGDA) (MW 10000) is a versatile polymer material that is widely used as a building material for hydrogel scaffolds, especially in the fields of tissue engineering and regenerative medicine. Poly(ethylene glycol) diacrylate (MW 10000) can be used to create a three-dimensional (3D) breast cancer cell culture model.

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Poly(ethylene glycol) diacrylate (MW 10000)

Poly(ethylene glycol) diacrylate (MW 10000) 화학구조

CAS No. : 26570-48-9

사이즈 가격 재고 수량
100 mg 해외재고보유
250 mg 해외재고보유
500 mg 해외재고보유
1 g 해외재고보유
5 g   견적 받기  
10 g   견적 받기  

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Poly(ethylene glycol) diacrylate (MW 10000) Related Antibodies

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제품 설명

Poly(ethylene glycol) diacrylate (PEGDA) (MW 10000) is a versatile polymer material that is widely used as a building material for hydrogel scaffolds, especially in the fields of tissue engineering and regenerative medicine. Poly(ethylene glycol) diacrylate (MW 10000) can be used to create a three-dimensional (3D) breast cancer cell culture model[1][2].

In Vitro

Poly(ethylene glycol) diacrylate (MW 10000) yields hydrogels that facilitate the 3D culture of breast cancer cells (MCF7, SK-BR-3, MDA-MB-231) and can be used to investigate the behavioral responses of cells under different matrix properties[2].

Product Properties:
This product is readily soluble in water and can be prepared into hydrogel precursor solutions of varying concentrations. Adding the photoinitiator LAP (HY-44076) to this precursor solution triggers crosslinking upon illumination, yielding a hydrogel with a certain mechanical strength.
Instructions:
1. Dissolve this product at a concentration of 10-20 (w/w)% in pure water or phosphate buffer at room temperature, depending on the intended use. Add 0.1-0.3% (w/w)% LAP photoinitiator to prepare a gel precursor solution.
2. Add the above solution to the mold and refrigerate. Irradiation under 405 nm light for 30-60 seconds will form PEGDA hydrogel.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Poly(ethylene glycol) diacrylate (MW 10000) Related Antibodies
분자량

10000.00 (Average)

화학식

(C2H4O)nC6H6O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(C=C)OCCOC(C=C)=O.[n]
선적

Room temperature in continental US; may vary elsewhere.
보관
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
용액&용해도
In Vitro: 

H2O : 100 mg/mL (Need ultrasonic)

DMSO : 50 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

  • 몰농도 계산기

  • 농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 5 mg/mL; Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 5 mg/mL; Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
순도&문서
References
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Poly(ethylene glycol) diacrylate (MW 10000) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Poly(ethylene glycol) diacrylate (MW 10000)26570-48-9PEGDA (MW 10000)Biochemical Assay ReagentsInhibitorinhibitorinhibit

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상품명:
Poly(ethylene glycol) diacrylate (MW 10000)
Cat. No.:
HY-W110542H
수량:
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