A selective inhibitor Gal-PUGNAc of human lysosomal beta-hexosaminidases modulates levels of the ganglioside GM2 in neuroblastoma cells

  • Angew Chem Int Ed Engl. 2009;48(7):1300-3. doi: 10.1002/anie.200804583.
Keith A Stubbs  1 ,  Matthew S Macauley ,  David J Vocadlo
Affiliations
  • 1. Department of Chemistry, Simon Fraser University, 8888 University Drive, Burnaby, British Columbia V5A 1S6, Canada. [email protected]
PMID: 19130519 DOI: 10.1002/anie.200804583
Abstract

Gal-PUGNAc (see picture), a highly selective inhibitor for beta-hexosaminidases HEXA and HEXB is cell-permeable and modulates the activity of HEXA and HEXB in tissue culture, increasing ganglioside GM2 levels. Gal-PUGNAc should allow the role of these Enzymes to be studied at the cellular level without generating a complex chemical phenotype from concomitant inhibition of O-GlcNAcase.

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