Protective effect of tea polyphenols against paracetamol-induced hepatotoxicity in mice is significantly correlated with cytochrome P450 suppression

  • World J Gastroenterol. 2009 Apr 21;15(15):1829-35. doi: 10.3748/wjg.15.1829.
Xia Chen  1 ,  Chang-Kai Sun ,  Guo-Zhu Han ,  Jin-Yong Peng ,  Ying Li ,  Yan-Xia Liu ,  Yuan-Yuan Lv ,  Ke-Xin Liu ,  Qin Zhou ,  Hui-Jun Sun
Affiliations
  • 1. Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, Lvshunkou District, Dalian, Liaoning Province, China.
PMID: 19370779 DOI: 10.3748/wjg.15.1829
Abstract

Aim: To investigate the hepatoprotective activity of tea Polyphenols (TP) and its relation with Cytochrome P450 (CYP450) expression in mice.

Methods: Hepatic CYP450 and CYPb(5) levels were measured by UV-spectrophotometry in mice 2 d after intraperitoneal TP (25, 50 and 100 mg/kg per day). Then the mice were intragastricly pre-treated with TP (100, 200 and 400 mg/kg per day) for six days before paracetamol (1000 mg/kg) was given. Their acute mortality was compared with that of control mice. The mice were pre-treated with TP (100, 200, and 400 mg/kg per day) for five days before paracetamol (500 mg/kg) was given. Hepatic CYP2E1 and CYP1A2 protein and mRNA expression levels were evaluated by Western blotting, immunohistochemical staining and transcriptase-polymerase chain reaction.

Results: The hepatic CYP450 and CYPb(5) levels in mice of TP-treated groups (100, 200 and 400 mg/kg per day) were decreased in a dose-dependent manner compared with those in the negative control mice. TP significantly attenuated the paracetamol-induced hepatic injury and dramatically reduced the mortality of paracetamol-treated mice. Furthermore, TP reduced CYP2E1 and CYP1A2 expression at both protein and mRNA levels in a dose-dependent manner.

Conclusion: TP possess potential hepatoprotective properties and can suppress CYP450 expression.

Products