Highly efficient chemoenzymatic synthesis of β1-3-linked galactosides

  • Chem Commun (Camb). 2010 Oct 28;46(40):7507-9. doi: 10.1039/c0cc02850a.
Hai Yu  1 ,  Vireak Thon ,  Kam Lau ,  Li Cai ,  Yi Chen ,  Shengmao Mu ,  Yanhong Li ,  Peng George Wang ,  Xi Chen
Affiliations
  • 1. Department of Chemistry, University of California, One Shields Avenue, Davis, California 95616, USA.
PMID: 20830443 DOI: 10.1039/c0cc02850a
Abstract

A novel D-galactosyl-β1-3-N-acetyl-D-hexosamine Phosphorylase cloned from Bifidobacterium infantis (BiGalHexNAcP) was used with a Recombinant E. coli K-12 Galactokinase (GalK) for efficient one-pot two-enzyme synthesis of T-antigens, galacto-N-biose (Galβ1-3GalNAc), lacto-N-biose (Galβ1-3GlcNAc), and their derivatives.

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