The characterisation of a galactokinase from Streptomyces coelicolor

  • Carbohydr Res. 2019 Jan 15;472:132-137. doi: 10.1016/j.carres.2018.12.005.
Tessa Keenan  1 ,  Rhys Mills  2 ,  Emily Pocock  2 ,  Darshita Budhadev  2 ,  Fabio Parmeggiani  3 ,  Sabine Flitsch  3 ,  Martin Fascione  2
Affiliations
  • 1. Department of Chemistry, University of York, Heslington, York, YO10 5DD, UK. Electronic address: [email protected].
  • 2. Department of Chemistry, University of York, Heslington, York, YO10 5DD, UK.
  • 3. Manchester Institute of Biotechnology, School of Chemistry, The University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK.
PMID: 30593944 DOI: 10.1016/j.carres.2018.12.005
Abstract

Promiscuous galactokinases (GalKs), which catalyse the ATP dependent phosphorylation of galactose in nature, have been widely exploited in biotechnology for the rapid synthesis of diverse sugar-1-phosphates. This work focuses on the characterisation of a Bacterial GalK from Streptomyces coelicolor (ScGalK), which was overproduced in Escherichia coli and shown to phosphorylate galactose. ScGalK displayed a broad substrate tolerance, with activity towards Gal, GalN, Gal3D, GalNAc, Man and L-Ara. Most interestingly, ScGalK demonstrated a high activity over a broad pH and temperature range, suggesting that the enzyme could be highly amenable to multi-enzyme systems.

Keywords
Galactokinase; Streptomyces coelicolor; Substrate specificity; Sugar-1-phosphates.
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