ABL1 Protein, Human (Active, sf9, His-StrepII)
ABL1 is a non-receptor tyrosine protein kinase that coordinates key cellular processes for growth and survival. It affects cytoskeletal remodeling, cell motility, and receptor endocytosis. ABL1 Protein, Human (Active, sf9, His-StrepII) is the recombinant human-derived ABL1, expressed by Sf9 insect cells, with N-8*His, N-StrepⅡ tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
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Biological Activity
Description
ABL1 is a non-receptor tyrosine protein kinase that coordinates key cellular processes for growth and survival. It affects cytoskeletal remodeling, cell motility, and receptor endocytosis. ABL1 Protein, Human (Active, sf9, His-StrepII) is the recombinant human-derived ABL1, expressed by Sf9 insect cells, with N-8*His, N-StrepⅡ tag.
Background
ABL1, a non-receptor tyrosine-protein kinase, orchestrates diverse cellular processes crucial for growth and survival. Its multifaceted roles include coordinating cytoskeleton remodeling in response to extracellular cues, regulating cell motility, and influencing receptor endocytosis. ABL1's impact extends to autophagy, DNA damage response, and apoptosis, where it phosphorylates key substrates involved in these pathways. Notably, it modulates actin dynamics by phosphorylating proteins like WASF3 and ANXA1, influencing processes such as lamellipodia formation and cell migration. A critical player in cell adhesion and motility, ABL1 phosphorylates regulators like BCAR1 and CRK. Furthermore, it engages in the regulation of receptor tyrosine kinases, including the facilitation of EGFR endocytosis. ABL1's reach extends to mitochondrial function during oxidative stress, where it mediates dysfunction and cell death. Additionally, it translocates to the nucleus, contributing to DNA damage response, apoptosis, and the regulation of T-cell differentiation. ABL1's extensive substrate repertoire encompasses proteins involved in DNA repair and apoptosis, showcasing its central role in cellular homeostasis and response to external stimuli.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-8*His;N-StrepⅡ
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Accession
P00519-1 (E27-R1130)
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Gene ID25
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Protein Length
Full Length
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Synonyms
ABL1; BCR/ABL1 E1a2 Fusion Protein; Prev. ABL; Bcr/C-Abl Oncogene Protein; JTK7; Chimeric BCR::ABL1 Protein; P150; BCR::ABL1 Fusion Protein; C-ABL; BCR/ABL E8a2 Fusion; V-Abl Abelson Murine Leukemia Viral Oncogene Homolog 1; BCR/ABL1 Fusion; C-Abl Oncogene 1, Receptor Tyrosine Kinase; BCR-ABL1 P190; Abelson Tyrosine-Protein Kinase 1; ABL1 Protein; Tyrosine-Protein Kinase ABL1; BCR-ABL; Proto-Oncogene C-Abl; Bcr/Abl; Abelson Murine Leukemia Viral Oncogene Homolog 1; CHDSKM; ABL Protooncogene 1 Nonreceptor Tyrosine Kinase; C-ABL1; C-Abl Oncogene 1, Non-Receptor Tyrosine Kinase; V-Abl; Proto-Oncogene Tyrosine-Protein Kinase ABL1; C-Abl; ABL Proto-Oncogene 1, Non-Receptor Tyrosine Kinase; BCR/ABL1 Fusion Protein E3a1
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AA Sequence
EALQRPVASDFEPQGLSEAARWNSKENLLAGPSENDPNLFVALYDFVASGDNTLSITKGEKLRVLGYNHNGEWCEAQTKNGQGWVPSNYITPVNSLEKHSWYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSESRFNTLAELVHHHSTVADGLITTLHYPAPKRNKPTVYGVSPNYDKWEMERTDITMKHKLGGGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTREPPFYIITEFMTYGNLLDYLRECNRQEVNAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNPSDRPSFAEIHQAFETMFQESSISDEVEKELGKQGVRGAVSTLLQAPELPTKTRTSRRAAEHRDTTDVPEMPHSKGQGESDPLDHEPAVSPLLPRKERGPPEGGLNEDERLLPKDKKTNLFSALIKKKKKTAPTPPKRSSSFREMDGQPERRGAGEEEGRDISNGALAFTPLDTADPAKSPKPSNGAGVPNGALRESGGSGFRSPHLWKKSSTLTSSRLATGEEEGGGSSSKRFLRSCSASCVPHGAKDTEWRSVTLPRDLQSTGRQFDSSTFGGHKSEKPALPRKRAGENRSDQVTRGTVTPPPRLVKKNEEAADEVFKDIMESSPGSSPPNLTPKPLRRQVTVAPASGLPHKEEAGKGSALGTPAAAEPVTPTSKAGSGAPGGTSKGPAEESRVRRHKHSSESPGRDKGKLSRLKPAPPPPPAASAGKAGGKPSQSPSQEAAGEAVLGAKTKATS
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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