ABL1 Protein, Human (Active, sf9, GST)

ABL1 is a non-receptor tyrosine protein kinase that coordinates key cellular processes for growth and survival. It affects cytoskeletal remodeling, cell motility, and receptor endocytosis. ABL1 Protein, Human (sf9, GST) is the recombinant human-derived ABL1 protein, expressed by Sf9 insect cells , with N-GST labeled tag.

ABL1, a non-receptor tyrosine-protein kinase, orchestrates diverse cellular processes crucial for growth and survival. Its multifaceted roles include coordinating cytoskeleton remodeling in response to extracellular cues, regulating cell motility, and influencing receptor endocytosis. ABL1's impact extends to autophagy, DNA damage response, and apoptosis, where it phosphorylates key substrates involved in these pathways. Notably, it modulates actin dynamics by phosphorylating proteins like WASF3 and ANXA1, influencing processes such as lamellipodia formation and cell migration. A critical player in cell adhesion and motility, ABL1 phosphorylates regulators like BCAR1 and CRK. Furthermore, it engages in the regulation of receptor tyrosine kinases, including the facilitation of EGFR endocytosis. ABL1's reach extends to mitochondrial function during oxidative stress, where it mediates dysfunction and cell death. Additionally, it translocates to the nucleus, contributing to DNA damage response, apoptosis, and the regulation of T-cell differentiation. ABL1's extensive substrate repertoire encompasses proteins involved in DNA repair and apoptosis, showcasing its central role in cellular homeostasis and response to external stimuli.

The specific activity was determined to be >15 nmol/min/mg using synthetic Abl peptide (EAIYAAPFAKKK) as substrate.

Human

Sf9 insect cells

N-GST

P00519-2 (P137-S554)

25  [NCBI]

Partial

PVNSLEKHSWYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSESRFNTLAELVHHHSTVADGLITTLHYPAPKRNKPTVYGVSPNYDKWEMERTDITMKHKLGGGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTREPPFYIITEFMTYGNLLDYLRECNRQEVNAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNPSDRPSFAEIHQAFETMFQESSISDEVEKELGKQGVRGAVSTLLQAPELPTKTRTS

Approximately 65 kDa

• 
  ≥ 90%, as determined by reducing SDS-PAGE.

Solution

1.Supplied as a 0.22 μm filtered solution of 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 0.5 mM EDTA, 0.5 mM Reduced Glutathione, pH 8.0.
2.Supplied as a 0.22 μm filtered solution of 20 mM Tris, 300 mM NaCl, 25% glycerol, 0.1 mM PMSF, 0.1 mM EDTA, 0.5 mM GSH, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.