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ABP1/AOC1 Protein, Human (HEK293, His)

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Based on 1 publication(s) in Google Scholar

ABP1/AOC1 Protein, Human (HEK293, His), a recombinant human Amiloride-binding protein 1 produced in HEK293 cells, has a His tag at the C-terminus. Amiloride-binding protein 1 is a membrane-associated amine oxidase and mainly metabolizes histamine and putrescine.

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  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for ABP1/AOC1 Protein, Human (HEK293, His)
  • ABP1/AOC1 Protein, Human (HEK293, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

ABP1/AOC1 Protein, Human (HEK293, His), a recombinant human Amiloride-binding protein 1 produced in HEK293 cells, has a His tag at the C-terminus. Amiloride-binding protein 1 is a membrane-associated amine oxidase and mainly metabolizes histamine and putrescine[1].

Background

Amiloride-binding protein 1 (Abp1), also called diamine oxidase, is a membrane-associated amine oxidase and mainly metabolizes histamine and putrescine. Plasma Abp1 has been proposed to be of value as an indicator of fetoplacental integrity[1].

Verified Bioactivity

Measured by its ability to produce hydrogen peroxide during the oxidation of histamine. The specific activity is 168.23 pmol/min/μg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    AAH14093.1 (E20-V751)

  • Gene ID
    26  [NCBI]
  • Molecular Construction
    • N-term
    • ABP1 (E20-V751)
      Accession # P19801
    • 6*His
    • C-term
  • Protein Length

    Full Length of Spondin-2 Chain

  • Synonyms

    AOC1; Amiloride-Binding Protein 1; Prev. ABP1; Histaminase; Prev. ABP; KDAO; DAO; KAO; Diamine Oxidase; Amiloride-Sensitive Amine Oxidase; Amiloride Binding Protein 1 (Amine Oxidase (Copper-Containing)); Amiloride-Binding Protein; Amine Oxidase Copper Dom

  • AA Sequence

    EPSPGTLPRKAGVFSDLSNQELKAVHSFLWSKKELRLQPSSTTTMAKNTVFLIEMLLPKKYHVLRFLDKGERHPVREARAVIFFGDQEHPNVTEFAVGPLPGPCYMRALSPRPGYQSSWASRPISTAEYALLYHTLQEATKPLHQFFLNTTGFSFQDCHDRCLAFTDVAPRGVASGQRRSWLIIQRYVEGYFLHPTGLELLVDHGSTDAGHWAVEQVWYNGKFYGSPEELARKYADGEVDVVVLEDPLPGGKGHDSTEEPPLFSSHKPRGDFPSPIHVSGPRLVQPHGPRFRLEGNAVLYGGWSFAFRLRSSSGLQVLNVHFGGERIAYEVSVQEAVALYGGHTPAGMQTKYLDVGWGLGSVTHELAPGIDCPETATFLDTFHYYDADDPVHYPRALCLFEMPTGVPLRRHFNSNFKGGFNFYAGLKGQVLVLRTTSTVYNYDYIWDFIFYPNGVMEAKMHATGYVHATFYTPEGLRHGTRLHTHLIGNIHTHLVHYRVDLDVAGTKNSFQTLQMKLENITNPWSPRHRVVQPTLEQTQYSWERQAAFRFKRKLPKYLLFTSPQENPWGHKRSYRLQIHSMADQVLPPGWQEEQAITWARYPLAVTKYRESELCSSSIYHQNDPWDPPVVFEQFLHNNENIENEDLVAWVTVGFLHIPHSEDIPNTATPGNSVGFLLRPFNFFPEDPSLASRDTVIVWPRDNGPNYVQRWIPEDRDCSMPPPFSYNGTYRPV

  • Molecular Weight

    Approximately 90 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

1.Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 10% glycerol, pH 7.5.
2.Supplied as a 0.22 μm filtered solution of 20 mM HEPES, 150 mM NaCl, pH 7.4, 10% glycerol.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

References

[1]. Liang XH, et al. Estrogen regulates amiloride-binding protein 1 through CCAAT/enhancer-binding protein-beta in mouse uterus during embryo implantation and decidualization. Endocrinology. 2010 Oct;151(10):5007-16. [Content Brief]

Calculators

Reconstitution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • ABP1/AOC1 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for ABP1/AOC1 Protein, Human (HEK293, His)
  • ABP1/AOC1 Protein, Human (HEK293, His) Product Image