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ADAM17/TACE Protein, Mouse (HEK293, His)

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The ADAM17/TACE protein cleaves TNF-α, releasing it from the membrane and activating various cell surface proteins such as JAM3, ACE2, and the p75 TNF receptor. It also triggers the Notch pathway by cleaving Notch into membrane-associated fragments. ADAM17/TACE Protein, Mouse (HEK293, His) is the recombinant mouse-derived ADAM17/TACE protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for ADAM17/TACE Protein, Mouse (HEK293, His)
  • ADAM17/TACE Protein, Mouse (HEK293, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The ADAM17/TACE protein cleaves TNF-α, releasing it from the membrane and activating various cell surface proteins such as JAM3, ACE2, and the p75 TNF receptor. It also triggers the Notch pathway by cleaving Notch into membrane-associated fragments. ADAM17/TACE Protein, Mouse (HEK293, His) is the recombinant mouse-derived ADAM17/TACE protein, expressed by HEK293 , with C-His labeled tag.

Background

ADAM17/TACE Protein is responsible for cleaving the membrane-bound precursor of TNF-alpha, converting it into its mature soluble form. It also plays a crucial role in the proteolytic release of various cell-surface proteins, including JAM3, ACE2, p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1, and the amyloid precursor protein. Additionally, ADAM17/TACE activates the Notch pathway by mediating the cleavage of Notch, generating a membrane-associated intermediate fragment known as notch extracellular truncation (NEXT). In hemostasis, it contributes by shedding the platelet glycoprotein Ib alpha chain (GP1BA), while also facilitating the proteolytic cleavage and release of secreted forms of LAG3 and IL6R. Furthermore, ADAM17/TACE mediates the proteolytic cleavage and shedding of FCGR3A upon stimulation of natural killer cells, allowing for enhanced NK cell motility and detachment from opsonized target cells.

Verified Bioactivity

Measured by its ability to cleave 10μM fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2 that incubate at room temperature in kinetic mode for 5 minutes. The specific activity is 1818.15 pmol/min/µg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q9Z0F8 (P18-D563)

  • Gene ID
    11491  [NCBI]
  • Molecular Construction
    • N-term
    • ADAM17 (P18-D563)
      Accession # Q9Z0F8
    • 6*His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    ADAM17; Snake Venom-Like Protease; Prev. TACE; ADAM Metallopeptidase Domain 18; CSVP; Uncharacterized Protein ADAM17; Disintegrin And Metalloproteinase Domain-Containing Protein 17; TNF-Alpha Converting Enzyme; TNF-Alpha Convertase; CD156b Antigen; CD156B

  • AA Sequence

    PRPPEEAGSGSHPRLEKLDSLLSDYDILSLANIQQHSIRKRDLQSATHLETLLTFSALKRHFKLYLTSSTERFSQNLRVVVVDGKEESEYSVKWQNFFSGHVVGEPDSRVLAHIGDDDVTVRINTDGAEYNVEPLWRFVNDTKDKRMLVYKSEDIKDFSRLQSPKVCGYLNADSEELLPKGLIDREPSEEFVRRVKRRAEPNPLKNTCKLLVVADHRFYKYMGRGEESTTTNYLIELIDRVDDIYRNTSWDNAGFKGYGVQIEQIRILKSPQEVKPGERHFNMAKSFPNEEKDAWDVKMLLEQFSFDIAEEASKVCLAHLFTYQDFDMGTLGLAYVGSPRANSHGGVCPKAYYNPTVKKNIYLNSGLTSTKNYGKTILTKEADLVTTHELGHNFGAEHDPDGLAECAPNEDQGGKYVMYPIAVSGDHENNKMFSNCSKQSIYKTIESKAQECFQERSNKVCGNSRVDEGEECDPGIMYLNNDTCCNSDCTLKPGVQCSDRNSPCCKNCQFETAQKKCQEAINATCKGVSYCTGNSSECPPPGDAED

  • Molecular Weight

    Approximately 19 kDa & 20 kDa (Propeptide) & 55-66 kDa (Mature-ADAM17) & 75-94 kDa (Pro-ADAM17), based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • ADAM17/TACE Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for ADAM17/TACE Protein, Mouse (HEK293, His)
  • ADAM17/TACE Protein, Mouse (HEK293, His) Product Image