ADH7 Protein, Human (HEK293, His)

ADH7 Protein, Human (HEK293, His) is human recombinant ADH7 with His tag at the C-terminus. ADH7 Protein, Human (HEK293, His) is expressed by Mammalian expression system and the target gene encoding Met1-Phe386 is expressed.

ADH7 is a NADPH-dependent enzyme belongs to the superfamily of medium-chain alcohol dehydrogenase. ADH7 is regulated by miR-3065, and miR-3065 interacted with LINC01133. ADH7 is enriched in the retinoic acid metabolic process and the retinol metabolism pathway. ADH7 is significantly associated with the cervical cancer survival rate^[1][2].

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

P40394 (M1-F386)

131  [NCBI]

Full Length

MFAEIQIQDKDRMGTAGKVIKCKAAVLWEQKQPFSIEEIEVAPPKTKEVRIKILATGICRTDDHVIKGTMVSKFPVIVGHEATGIVESIGEGVTTVKPGDKVIPLFLPQCRECNACRNPDGNLCIRSDITGRGVLADGTTRFTCKGKPVHHFMNTSTFTEYTVVDESSVAKIDDAAPPEKVCLIGCGFSTGYGAAVKTGKVKPGSTCVVFGLGGVGLSVIMGCKSAGASRIIGIDLNKDKFEKAMAVGATECISPKDSTKPISEVLSEMTGNNVGYTFEVIGHLETMIDALASCHMNYGTSVVVGVPPSAKMLTYDPMLLFTGRTWKGCVFGGLKSRDDVPKLVTEFLAKKFDLDQLITHVLPFKKISEGFELLNSGQSIRTVLTF

Approximately 44 kDa,based on SDS-PAGE under reducing conditions.

≥ 80%, as determined by reducing SDS-PAGE.

Lyophilized powder

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 8% trehalose, 4% Mannitol, 0.02% Twenn80, pH 6.9.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Ding S, et al. ADH7, miR-3065 and LINC01133 are associated with cervical cancer progression in different age groups. Oncol Lett. 2020 Mar;19(3):2326-2338.  [Content Brief]

  [2]. Nguyen TT, et al. The ADH7 Promoter of Saccharomyces cerevisiae is Vanillin-Inducible and Enables mRNA Translation Under Severe Vanillin Stress. Front Microbiol. 2015 Dec 11;6:1390.  [Content Brief]