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  3. Enzymes & Regulators
  4. Oxidoreductases (EC 1)
  5. ADH7 Protein, Human (HEK293, His)

ADH7 Protein, Human (HEK293, His)

Cat. No.: HY-P7473
Handling Instructions

ADH7 Protein, Human (HEK293, His) is human recombinant ADH7 with His tag at the C-terminus. ADH7 Protein, Human (HEK293, His) is expressed by Mammalian expression system and the target gene encoding Met1-Phe386 is expressed.

For research use only. We do not sell to patients.

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Description

ADH7 Protein, Human (HEK293, His) is human recombinant ADH7 with His tag at the C-terminus. ADH7 Protein, Human (HEK293, His) is expressed by Mammalian expression system and the target gene encoding Met1-Phe386 is expressed.
Background

ADH7 is a NADPH-dependent enzyme belongs to the superfamily of medium-chain alcohol dehydrogenase. ADH7 is regulated by miR-3065, and miR-3065 interacted with LINC01133. ADH7 is enriched in the retinoic acid metabolic process and the retinol metabolism pathway. ADH7 is significantly associated with the cervical cancer survival rate[1][2].
Species

Human
Source

HEK293
Tag

C-6*His
Accession

P40394 (M1-F386)
Gene ID

131  [NCBI]
Molecular Construction
N-term
ADH7 (M1-F386)
Accession # P40394
6*His
C-term
Synonyms
rHuADH7, His; Alcohol dehydrogenase class 4 mu, sigma chain; ADH-7
AA Sequence

MFAEIQIQDKDRMGTAGKVIKCKAAVLWEQKQPFSIEEIEVAPPKTKEVRIKILATGICRTDDHVIKGTMVSKFPVIVGHEATGIVESIGEGVTTVKPGDKVIPLFLPQCRECNACRNPDGNLCIRSDITGRGVLADGTTRFTCKGKPVHHFMNTSTFTEYTVVDESSVAKIDDAAPPEKVCLIGCGFSTGYGAAVKTGKVKPGSTCVVFGLGGVGLSVIMGCKSAGASRIIGIDLNKDKFEKAMAVGATECISPKDSTKPISEVLSEMTGNNVGYTFEVIGHLETMIDALASCHMNYGTSVVVGVPPSAKMLTYDPMLLFTGRTWKGCVFGGLKSRDDVPKLVTEFLAKKFDLDQLITHVLPFKKISEGFELLNSGQSIRTVLTFHHHHHHHH

Molecular Weight

Approximately 44.0 kDa
Purity

Greater than 80% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)
References

ADH7 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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