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  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. ADK/Adenosine Kinase Protein, Human (HEK293)

ADK/Adenosine Kinase Protein, Human (HEK293)

Cat. No.: HY-P75530
COA Handling Instructions

ADK is an enzyme that catalyzes the transfer of gamma-phosphate from adenosine triphosphate (ATP) to adenosine (Ado) to form adenosine monophosphate (AMP). ADK acts as a potential regulator of extracellular adenosine and intracellular adenine nucleotide concentrations. ADK is a therapeutic target for controlling obesity and non-alcoholic fatty liver disease. ADK/Adenosine Kinase Protein, Human (HEK293) is the recombinant human-derived ADK/Adenosine Kinase protein, expressed by HEK293 , with tag free. The total length of ADK/Adenosine Kinase Protein, Human (HEK293) is 345 a.a., with molecular weight of ~38.74 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

ADK is an enzyme that catalyzes the transfer of gamma-phosphate from adenosine triphosphate (ATP) to adenosine (Ado) to form adenosine monophosphate (AMP). ADK acts as a potential regulator of extracellular adenosine and intracellular adenine nucleotide concentrations. ADK is a therapeutic target for controlling obesity and non-alcoholic fatty liver disease. ADK/Adenosine Kinase Protein, Human (HEK293) is the recombinant human-derived ADK/Adenosine Kinase protein, expressed by HEK293 , with tag free. The total length of ADK/Adenosine Kinase Protein, Human (HEK293) is 345 a.a., with molecular weight of ~38.74 kDa.

Background

Adenosine kinase is an enzyme that catalyzes the transfer of γ-phosphate from adenosine triphosphate (ATP) to adenosine (Ado) to form adenosine monophosphate (AMP). Catalyze the phosphorylation of purine nucleoside adenosine at the 5 'position in an ATP-dependent manner. ADK acts as a potential regulator of extracellular adenosine and intracellular adenine nucleotide concentrations. ADK promotes excess fat deposition and liver inflammation by inhibiting fatty acid oxidation in hepatocytes and producing hepatocellular proinflammatory mediators, which is a therapeutic target for controlling obesity and non-alcoholic fatty liver disease[1][2][3].

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

HEK293

Tag

Tag Free

Accession

AAH03568 (M1-H345)

Gene ID

132  [NCBI]

Molecular Construction
N-term
ADK (M1-H345)
Accession # AAH03568
C-term
Synonyms
Adenosine kinase; AK; Adenosine 5'-phosphotransferase; ADK
Molecular Weight

Approximately 38.74 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 300 mM NaCl pH 8.0, 0.5 mM PMSF, 0.5 mM TCEP, 20% glycerol.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

ADK/Adenosine Kinase Protein, Human (HEK293) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ADK/Adenosine Kinase Protein, Human (HEK293)
Cat. No.:
HY-P75530
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