2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Hydrolases (EC 3) Phosphatase
  5. Alkaline Phosphatase
  6. Alkaline Phosphatase/ALPL Protein, Mouse (HEK293, C-His)

Alkaline Phosphatase/ALPL Protein, Mouse (HEK293, C-His)

Cat. No.: HY-P700653
Handling Instructions

Alkaline Phosphatase/ALPL Protein, vital with phosphatase, metal ion binding, and homodimerization activities, influences BMP stimulus response. Strategically located externally on the plasma membrane, its predicted activity emphasizes cellular functions. Prominent expression in the intestine and testis, especially in duodenum adult (RPKM 2234.3) and small intestine adult (RPKM 1574.5), underscores specific roles in these contexts. Its human orthologs, implicated in inflammatory bowel disease, highlight clinical relevance. Evolutionarily conserved, Alkaline Phosphatase/ALPL shares orthologous counterparts like ALPI in several human genes. Alkaline Phosphatase/ALPL Protein, Mouse (HEK293, C-His) is the recombinant mouse-derived Alkaline Phosphatase/ALPL protein, expressed by HEK293 , with C-His labeled tag. The total length of Alkaline Phosphatase/ALPL Protein, Mouse (HEK293, C-His) is 481 a.a., with molecular weight of 60-70 kDa.

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Description

Alkaline Phosphatase/ALPL Protein, vital with phosphatase, metal ion binding, and homodimerization activities, influences BMP stimulus response. Strategically located externally on the plasma membrane, its predicted activity emphasizes cellular functions. Prominent expression in the intestine and testis, especially in duodenum adult (RPKM 2234.3) and small intestine adult (RPKM 1574.5), underscores specific roles in these contexts. Its human orthologs, implicated in inflammatory bowel disease, highlight clinical relevance. Evolutionarily conserved, Alkaline Phosphatase/ALPL shares orthologous counterparts like ALPI in several human genes. Alkaline Phosphatase/ALPL Protein, Mouse (HEK293, C-His) is the recombinant mouse-derived Alkaline Phosphatase/ALPL protein, expressed by HEK293 , with C-His labeled tag. The total length of Alkaline Phosphatase/ALPL Protein, Mouse (HEK293, C-His) is 481 a.a., with molecular weight of 60-70 kDa.
Background

Alkaline Phosphatase/ALPL Protein is predicted to play several vital roles, including alkaline phosphatase activity, metal ion binding activity, and protein homodimerization activity. It functions upstream of or within the cellular response to BMP stimulus, suggesting its involvement in critical signaling pathways. The protein is predicted to be strategically located in the external side of the plasma membrane, indicating its role in extracellular processes. Its predicted activity in the plasma membrane further emphasizes its significance in cellular functions. This protein is expressed prominently in the intestine and testis, and the biased expression profile in duodenum adult (RPKM 2234.3), small intestine adult (RPKM 1574.5), and one other tissue underscores its specific roles in these physiological contexts. The human ortholog(s) of this gene have been implicated in inflammatory bowel disease, further highlighting its clinical relevance. Alkaline Phosphatase/ALPL is evolutionarily conserved, with orthologous counterparts such as ALPI (alkaline phosphatase, intestinal) in several human genes.
Species

Mouse
Source

HEK293
Tag

C-His
Accession

NP_001074551.1 (I21-G501)
Gene ID

76768  [NCBI]
Molecular Construction
N-term
ALPL (I21-G501)
Accession # NP_001074551.1
His
C-term
Synonyms
IAP; Akp2; ALPL; APTNAP; AP-TNAP; FLJ40094; FLJ93059; HOPS; MGC161443; TNAP; TNSALP
Molecular Weight

60-70 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

Alkaline Phosphatase/ALPL Protein, Mouse (HEK293, C-His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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