Angiogenin Protein, Human (His)
Human angiopoietin protein is a ribonuclease that cleaves tRNA to generate stress-induced fragments, inhibits protein synthesis and triggers stress granule assembly. It binds to endothelial cell actin, undergoes endocytosis and nuclear translocation, and stimulates ribosomal RNA synthesis. Angiogenin Protein, Human (His) is the recombinant human-derived Angiogenin protein,Human, expressed by E. coli , with N-6*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Human angiopoietin protein is a ribonuclease that cleaves tRNA to generate stress-induced fragments, inhibits protein synthesis and triggers stress granule assembly. It binds to endothelial cell actin, undergoes endocytosis and nuclear translocation, and stimulates ribosomal RNA synthesis. Angiogenin Protein, Human (His) is the recombinant human-derived Angiogenin protein,Human, expressed by E. coli , with N-6*His labeled tag.
Angiogenin protein, a ribonuclease, demonstrates the ability to cleave tRNA within anticodon loops, producing tRNA-derived stress-induced fragments (tiRNAs). These tiRNAs play a crucial role in inhibiting protein synthesis and triggering the assembly of stress granules (SGs). Additionally, Angiogenin binds to actin on the surface of endothelial cells, and upon binding, it is endocytosed and translocated to the nucleus. In the nucleus, Angiogenin stimulates ribosomal RNA synthesis, including the initiation site sequences of 45S rRNA. Furthermore, Angiogenin exhibits angiogenic activity, promoting vascularization in both normal and malignant tissues. This angiogenic function is intricately regulated by its interaction with RNH1 in vivo. Structurally, Angiogenin forms homodimers and interacts with RNH1 to create tight 1:1 complexes, with the possibility of dimerization involving two such complexes.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His
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Accession
P03950 (D26-P147)
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Molecular Construction
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N-term
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6*His
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Angiogenin (D26-P147)
Accession # P03950 -
C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
ANG; Angiogenin, Ribonuclease, RNase A Family, 5; Angiogenin; Ribonuclease A Family Member 5; RNASE5; RNase 5; Ribonuclease 5; Epididymis Luminal Protein 168; HEL168; Ribonuclease A A1; RAA1; RNASE4; Homo Sapiens Epididymis Luminal Protein 168; ALS9
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AA Sequence
DNSRYTHFLTQHYDAKPQGRDDRYCESIMRRRGLTSPCKDINTFIHGNKRSIKAICENKNGNPHRENLRISKSSFQVTTCKLHGGSPWPPCQYRATAGFRNVVVACENGLPVHLDQSIFRRP
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Predicted Molecular Mass
18.0 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/ug, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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