Animal-Free BMP-2 Protein, Human/Mouse/Rat (His)

BMP-2 protein is an important member of the TGF-β superfamily and is critical in cardiogenesis, neurogenesis, and osteogenesis, inducing cartilage and bone formation. It initiates canonical BMP signaling by binding to BMPR1A and BMPR2, triggering BMPR2 phosphorylation and SMAD1/5/8 activation for gene transcription regulation. Animal-Free BMP-2 Protein, Human/Mouse/Rat (His) is the recombinant human-derived animal-Free BMP-2 protein, expressed by E. coli , with C-His labeled tag.This product is for cell culture use only.

BMP-2 Protein, a vital member of the TGF-beta superfamily, plays essential roles in diverse developmental processes, including cardiogenesis, neurogenesis, and osteogenesis. It induces cartilage and bone formation and initiates the canonical BMP signaling cascade by binding to type I receptor BMPR1A and type II receptor BMPR2. This complex formation triggers BMPR2 phosphorylation, activating BMPR1A, which, in turn, phosphorylates SMAD1/5/8 to modulate gene transcription. BMP-2 also engages non-canonical pathways, such as the ERK/MAP kinase signaling cascade, influencing osteoblast differentiation. Additionally, it stimulates myoblast differentiation into osteoblasts through the EIF2AK3-EIF2A-ATF4 pathway. Acting as a positive regulator of odontoblast differentiation, BMP-2 forms homodimers and interacts with various proteins, including SOSTDC1, GREM2, RGMA, RGMB, RGMC, ASPN, FBN1, FBN2, SCUBE3, TNFAIP6, and ERFE. Its intricate interactions highlight its versatile regulatory roles in multiple cellular processes.

Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is <9.5 ng/mL. The specific activity of recombinant BMP-2 is > 3.2 x 10⁶ IU/mg

Human

E. coli

C-6*His

P12643 (Q283-R396)

650  [NCBI]

Full Length of Mature Protein

QAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQTLVNSVNSKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCR

Approximately 14 kDa, based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM sodium citrate, 0.2 M NaCl, pH 3.5, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is recommended to reconstitute to a concentration of 100-200 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.