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  2. Cytokines and Growth Factors Immune Checkpoint Proteins CD Antigens Animal-free Recombinant Proteins
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  4. TNF Superfamily Ligands CD40 Ligand/CD154
  5. CD40 Ligand
  6. Animal-Free CD40L/CD154/TRAP Protein, Human (His)

Animal-Free CD40L/CD154/TRAP Protein, Human (His)

Cat. No.: HY-P72907AF
COA Handling Instructions

CD40L/CD154/TRAP proteins stimulate T cell proliferation and cytokine production, acting as ligands for integrins and CD40 receptors to activate various cell-dependent effects such as B cell activation and NF-κ-B signaling. Animal-Free CD40L/CD154/TRAP Protein, Human (His) is the recombinant human-derived animal-FreeCD40L/CD154/TRAP protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free CD40L/CD154/TRAP Protein, Human (His) is 154 a.a., with molecular weight of ~16.99 kDa.

Animal-free recombinant proteins offer high consistency and stability, whithout using or contacting of any animal-derived materials and reagents.

For research use only. We do not sell to patients.

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Description

CD40L/CD154/TRAP proteins stimulate T cell proliferation and cytokine production, acting as ligands for integrins and CD40 receptors to activate various cell-dependent effects such as B cell activation and NF-κ-B signaling. Animal-Free CD40L/CD154/TRAP Protein, Human (His) is the recombinant human-derived animal-FreeCD40L/CD154/TRAP protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free CD40L/CD154/TRAP Protein, Human (His) is 154 a.a., with molecular weight of ~16.99 kDa.

Background

CD40L, also known as CD154 or TRAP protein, functions as a cytokine and serves as a ligand for CD40/TNFRSF5, orchestrating a cascade of immunological responses. It plays a pivotal role in T-cell activation, acting as a potent co-stimulator that enhances both T-cell proliferation and cytokine production, specifically IL4 and IL10. Upon cross-linking on T-cells, CD40L generates a costimulatory signal synergizing with TCR/CD3 ligation and CD28 costimulation. Additionally, CD40L induces the activation of NF-kappa-B, triggers the activation of kinases MAPK8 and PAK2 in T-cells, and facilitates tyrosine phosphorylation of CD28 isoform 3. Beyond T-cell modulation, CD40L mediates B-cell proliferation in the absence of co-stimulus and promotes IgE production in the presence of IL4, contributing to immunoglobulin class switching. Furthermore, it serves as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3, and collaborates with the CD40 receptor in the activation of CD40-CD40LG signaling, exerting cell-type-dependent effects such as B-cell activation, NF-kappa-B signaling, and anti-apoptotic signaling.

Biological Activity

Measure by its ability to induce IL-8 secretion in human PBMCs. The ED50 for this effect is <5 ng/mL.

Species

Human

Source

E. coli

Tag

C-His

Accession

P29965 (E108-L261)

Gene ID

959  [NCBI]

Molecular Construction
N-term
CD40L (E108-L261)
Accession # P29965
His
C-term
Synonyms
soluble CD40 Ligand; CD40 ligand; CD40-L; TRAP; CD154; sCD40L; TNFSF5
AA Sequence

MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLWLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL

Molecular Weight

Approximately 16.99 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a solution containing 1X PBS, pH 8.0.

Endotoxin Level

<0.1 EU per 1 μg of the protein by the LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Animal-Free CD40L/CD154/TRAP Protein, Human (His)
Cat. No.:
HY-P72907AF
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