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Animal-Free FGF-6 Protein, Human (His)

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The FGF-6 protein coordinates cell proliferation, differentiation, angiogenesis, and myogenesis and is critical for normal muscle regeneration. It interacts with FGFR1, FGFR2, and FGFR4 to mediate signaling cascades that affect various cellular processes. Animal-Free FGF-6 Protein, Human (His) is the recombinant human-derived animal-FreeFGF-6 protein, expressed by E. coli , with C-His labeled tag. This product is for cell culture use only.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The FGF-6 protein coordinates cell proliferation, differentiation, angiogenesis, and myogenesis and is critical for normal muscle regeneration. It interacts with FGFR1, FGFR2, and FGFR4 to mediate signaling cascades that affect various cellular processes. Animal-Free FGF-6 Protein, Human (His) is the recombinant human-derived animal-FreeFGF-6 protein, expressed by E. coli , with C-His labeled tag. This product is for cell culture use only.

Background

FGF-6 Protein assumes a pivotal role in orchestrating the intricate regulation of cell proliferation, differentiation, angiogenesis, and myogenesis, while playing a crucial part in ensuring normal muscle regeneration. Its interactions with FGFR1, FGFR2, and FGFR4 underline its involvement in mediating signaling cascades that impact various cellular processes. Furthermore, the affinity between fibroblast growth factors (FGFs) and their receptors is heightened by the presence of heparan sulfate glycosaminoglycans, acting as coreceptors in this intricate signaling network. The multifaceted actions of FGF-6 Protein underscore its significance in coordinating essential cellular events, highlighting its role in tissue development, angiogenesis, and the intricate process of muscle regeneration.

Verified Bioactivity

Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is <0.1 ng/mL. The specific activity of recombinant human FGF-6 is >1 x 107IU/mg.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag C-6*His
  • Accession

    P10767 (G41-I208)

  • Gene ID
    2251  [NCBI]
  • Molecular Construction
    • N-term
    • FGF-6 (G41-I208)
      Accession # P10767
    • 6*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    FGF6; FGF-6; Fibroblast Growth Factor 6; HST-2; Heparin Secretory-Transforming Protein 2; HST2; Heparin-Binding Growth Factor 6; Fibroblast Growth Factor; HBGF-6; HSTF2; HSTF-2; FGF

  • AA Sequence

    GTRANNTLLDSRGWGTLLSRSRAGLAGEIAGVNWESGYLVGIKRQRRLYCNVGIGFHLQVLPDGRISGTHEENPYSLLEISTVERGVVSLFGVRSALFVAMNSKGRLYATPSFQEECKFRETLLPNNYNAYESDLYQGTYIALSKYGRVKRGSKVSPIMTVTHFLPRI

  • Predicted Molecular Mass

    19.7 kDa

  • Molecular Weight

    Approximately 19 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM sodium citrate, 0.2 M NaCl, pH 3.5, trehalose.

Endotoxin Level

<0.1 EU per 1 μg of the protein by the LAL method.

Reconstitution

It is recommended to reconstitute to a concentration of 100-200 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
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Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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