Animal-Free FGF-6 Protein, Human (His)

FGF-6 Protein orchestrates cell proliferation, differentiation, angiogenesis, and myogenesis, crucial for normal muscle regeneration. Interacting with FGFR1, FGFR2, and FGFR4, it mediates signaling cascades impacting various cellular processes. The presence of heparan sulfate glycosaminoglycans enhances FGF-6's affinity with fibroblast growth factors and receptors. FGF-6 Protein's multifaceted actions underscore its significance in coordinating essential cellular events, playing a vital role in tissue development, angiogenesis, and the intricate process of muscle regeneration. Animal-Free FGF-6 Protein, Human (His) is the recombinant human-derived animal-FreeFGF-6 protein, expressed by E. coli, with C-His labeled tag. The total length of Animal-Free FGF-6 Protein, Human (His) is 168 a.a., with molecular weight of ~19.66 kDa.

FGF-6 Protein assumes a pivotal role in orchestrating the intricate regulation of cell proliferation, differentiation, angiogenesis, and myogenesis, while playing a crucial part in ensuring normal muscle regeneration. Its interactions with FGFR1, FGFR2, and FGFR4 underline its involvement in mediating signaling cascades that impact various cellular processes. Furthermore, the affinity between fibroblast growth factors (FGFs) and their receptors is heightened by the presence of heparan sulfate glycosaminoglycans, acting as coreceptors in this intricate signaling network. The multifaceted actions of FGF-6 Protein underscore its significance in coordinating essential cellular events, highlighting its role in tissue development, angiogenesis, and the intricate process of muscle regeneration.

Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <0.1 ng/mL. The specific activity of recombinant human FGF-6 is >1 x 10⁷IU/mg.

Human

E. coli

C-His

P10767 (G41-I208)

2251  [NCBI]

MGTRANNTLLDSRGWGTLLSRSRAGLAGEIAGVNWESGYLVGIKRQRRLYCNVGIGFHLQVLPDGRISGTHEENPYSLLEISTVERGVVSLFGVRSALFVAMNSKGRLYATPSFQEECKFRETLLPNNYNAYESDLYQGTYIALSKYGRVKRGSKVSPIMTVTHFLPRI

Approximately 19.66 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a solution containing 20 mM sodium citrate, 0.2 MNaCl, pH 3.5.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.