Animal-Free FGF-6 Protein, Human (His)

The FGF-6 protein coordinates cell proliferation, differentiation, angiogenesis, and myogenesis and is critical for normal muscle regeneration. It interacts with FGFR1, FGFR2, and FGFR4 to mediate signaling cascades that affect various cellular processes. Animal-Free FGF-6 Protein, Human (His) is the recombinant human-derived animal-FreeFGF-6 protein, expressed by E. coli , with C-His labeled tag. This product is for cell culture use only.

FGF-6 Protein assumes a pivotal role in orchestrating the intricate regulation of cell proliferation, differentiation, angiogenesis, and myogenesis, while playing a crucial part in ensuring normal muscle regeneration. Its interactions with FGFR1, FGFR2, and FGFR4 underline its involvement in mediating signaling cascades that impact various cellular processes. Furthermore, the affinity between fibroblast growth factors (FGFs) and their receptors is heightened by the presence of heparan sulfate glycosaminoglycans, acting as coreceptors in this intricate signaling network. The multifaceted actions of FGF-6 Protein underscore its significance in coordinating essential cellular events, highlighting its role in tissue development, angiogenesis, and the intricate process of muscle regeneration.

Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <0.1 ng/mL. The specific activity of recombinant human FGF-6 is >1 x 10⁷IU/mg.

Human

E. coli

C-6*His

P10767 (G41-I208)

2251  [NCBI]

Full Length of Mature Protein

GTRANNTLLDSRGWGTLLSRSRAGLAGEIAGVNWESGYLVGIKRQRRLYCNVGIGFHLQVLPDGRISGTHEENPYSLLEISTVERGVVSLFGVRSALFVAMNSKGRLYATPSFQEECKFRETLLPNNYNAYESDLYQGTYIALSKYGRVKRGSKVSPIMTVTHFLPRI

Approximately 19 kDa, based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM sodium citrate, 0.2 M NaCl, pH 3.5, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is recommended to reconstitute to a concentration of 100-200 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.