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  2. Cytokines and Growth Factors Animal-free Recombinant Proteins
  3. Interferon & Receptors
  4. IFN-lambda
  5. IFN-lambda 3/IL-28B
  6. Animal-Free IFN-lambda 3/IL-28B Protein, Human (His)

Animal-Free IFN-lambda 3/IL-28B Protein, Human (His)

Cat. No.: HY-P700120AF
COA Handling Instructions

The IFN-lambda 3/IL-28B protein is a multifaceted cytokine with antiviral, antitumor, and immunomodulatory activities that plays a key role in antiviral host defense, particularly within epithelial tissues. Animal-Free IFN-lambda 3/IL-28B Protein, Human (His) is the recombinant human-derived animal-FreeIFN-lambda 3/IL-28B protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IFN-lambda 3/IL-28B Protein, Human (His) is 175 a.a., with molecular weight of ~25.57 kDa.

Animal-free recombinant proteins offer high consistency and stability, whithout using or contacting of any animal-derived materials and reagents.

For research use only. We do not sell to patients.

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Description

The IFN-lambda 3/IL-28B protein is a multifaceted cytokine with antiviral, antitumor, and immunomodulatory activities that plays a key role in antiviral host defense, particularly within epithelial tissues. Animal-Free IFN-lambda 3/IL-28B Protein, Human (His) is the recombinant human-derived animal-FreeIFN-lambda 3/IL-28B protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IFN-lambda 3/IL-28B Protein, Human (His) is 175 a.a., with molecular weight of ~25.57 kDa.

Background

IFN-lambda 3/IL-28B Protein, a multifaceted cytokine, exhibits antiviral, antitumor, and immunomodulatory activities, playing a pivotal role in antiviral host defense, particularly within epithelial tissues. Serving as a ligand for the heterodimeric class II cytokine receptor consisting of IL10RB and IFNLR1, its engagement initiates the JAK/STAT signaling pathway, leading to the expression of IFN-stimulated genes (ISG) that establish the antiviral state. Notably, its receptor distribution is constrained, primarily exerting effects in epithelial cells due to the cell type-specific expression of the receptor IFNLR1. While not considered essential for early virus-activated host defense in vaginal infection, it proves crucial in Toll-like receptor (TLR)-induced antiviral defense and significantly contributes to the antiviral immune defense in the intestinal epithelium. Additionally, IFN-lambda 3/IL-28B exerts an immunomodulatory effect by up-regulating MHC class I antigen expression, enhancing its impact on immune responses.

Biological Activity

Measure by its ability to protect HepG2 cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is <5 ng/mL.

Species

Human

Source

E. coli

Tag

C-His

Accession

Q8IZI9 (V22-V196)

Gene ID
Molecular Construction
N-term
IFN-λ3 (V22-V196)
Accession # Q8IZI9
His
C-term
Synonyms
Interferon lambda-3; IFN-lambda-3; IL-28B; IL-28C; ZCYTO22
AA Sequence

MVPVARLRGALPDARGCHIAQFKSLSPQELQAFKRAKDALEESLLLKDCKCRSRLFPRTWDLRQLQVRERPVALEAELALTLKVLEATADTDPALGDVLDQPLHTLHHILSQLRACIQPQPTAGPRTRGRLHHWLHRLQEAPKKESPGCLEASVTFNLFRLLTRDLNCVASGDLCV

Molecular Weight

Approximately 25.57 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a solution containing 1X PBS, pH 8.0.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Animal-Free IFN-lambda 3/IL-28B Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Animal-Free IFN-lambda 3/IL-28B Protein, Human (His)
Cat. No.:
HY-P700120AF
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