Anionic trypsin-1 Protein, Rat (His)

Serine protease 1 is a member of the trypsin family of serine proteases.Serine protease 1 is secreted by the pancreas and cleaved to its active form in the small intestine.Anionic trypsin-1 Protein, Rat (His) is the recombinant rat-derived Anionic trypsin-1 protein, expressed by E. coli , with N-10*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Rat
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs

Biological Activity

Description

Serine protease 1 is a member of the trypsin family of serine proteases.Serine protease 1 is secreted by the pancreas and cleaved to its active form in the small intestine.Anionic trypsin-1 Protein, Rat (His) is the recombinant rat-derived Anionic trypsin-1 protein, expressed by E. coli , with N-10*His labeled tag.

Background

Serine protease 1 is a member of the trypsin family of serine proteases. Serine protease 1 is secreted by the pancreas and cleaved to its active form in the small intestine. Serine protease 1 is active on peptide linkages involving the carboxyl group of lysine or arginine. Mutations in this gene are associated with hereditary pancreatitis. This gene and several other trypsinogen genes are localized to the T cell receptor beta locus on chromosome 7.

Technical Parameters

  • Species Rat
  • Source E. coli
  • Tag N-10*His
  • Accession
  • Gene ID
  • Molecular Construction
    • N-term
    • 10*His
    • Prss1 (24I–246N)
      Accession # P00762
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    PRSS1; TRP1; Prev. TRY1; Anionic Trypsin-I; Cationic Trypsinogen; Protease Serine 1; Nonfunctional Trypsin 1; TCR V Beta 4.1; Protease, Serine 1; Trypsinogen A; Anionic Trypsin I; Trypsinogen 1; Pretrypsinogen I; Trypsin 1; Beta-Trypsin; Trypsin-1; Trypsi

  • AA Sequence

    IVGGYTCPEHSVPYQVSLNSGYHFCGGSLINDQWVVSAAHCYKSRIQVRLGEHNINVLEGDEQFINAAKIIKHPNYSSWTLNNDIMLIKLSSPVKLNARVAPVALPSACAPAGTQCLISGWGNTLSNGVNNPDLLQCVDAPVLSQADCEAAYPGEITSSMICVGFLEGGKDSCQGDSGGPVVCNGQLQGIVSWGYGCALPDNPGVYTKVCNFVGWIQDTIAAN

  • Predicted Molecular Mass

    27.2 kDa

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
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Concentration (final) Concentration (final)
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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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