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Annexin A1/ANXA1 Protein, Human

2 Cited Publications
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Based on 2 publication(s) in Google Scholar

Annexin A1 Protein, Human is an approximately 34.0 kDa Annexin A1 protein expressed by the E. coli system. Annexin A1 is a Ca2+-dependent phospholipid binding protein and has inhibitory effects on phospholipase A2 (PLA2).

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  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Annexin A1/ANXA1 Protein, Human
  • Experimental Validation Results for Annexin A1/ANXA1 Protein, Human
  • Annexin A1/ANXA1 Protein, Human Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

Annexin A1 Protein, Human is an approximately 34.0 kDa Annexin A1 protein expressed by the E. coli system. Annexin A1 is a Ca2+-dependent phospholipid binding protein and has inhibitory effects on phospholipase A2 (PLA2)[1].

Background

Annexin A1 is a member of the Ca2+-dependent phospholipid binding proteins that are localized on the cytosolic face of the plasma membrane. Annexin A1 inhibits activity of phospholipase A2, binding 2-4 calcium ions with high affinity. Annexin A1 is a protein with multifunctional roles in innate and adaptive immunity mainly devoted to the regulation of inflammatory cells and the resolution of inflammation. Annexin A1 is an anti-inflammatory molecule playing major role as an inhibitor of neutrophil transmigration and recruitment at inflammatory sites[1][2].

Verified Bioactivity

Measured by its ability to inhibit proliferation of A549 human lung carcinoma cells. The ED50 for this effect is ≤2 μg/mL, corresponding to a specific activity is ≥500 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to inhibit proliferation of A549 human lung carcinoma cells. The ED50 for this effect is 0.7223 μg/mL, corresponding to a specific activity is 1.38×103 units/mg.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag Tag Free
  • Accession

    P04083 (A2-N346)

  • Gene ID
    301  [NCBI]
  • Molecular Construction
    • N-term
    • ANXA1 (A2-N346)
      Accession # P04083
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    ANXA1; Annexin-1; Prev. ANX1; Annexin I (Lipocortin I); Prev. LPC1; ANXA1 Protein; Phospholipase A2 Inhibitory Protein; Lipocortin I; Chromobindin-9; Annexin I; Calpactin II; Annexin; Calpactin-2; P35; Annexin A1; Epididymis Secretory Sperm Binding Protein

  • AA Sequence

    AMVSEFLKQAWFIENEEQEYVQTVKSSKGGPGSAVSPYPTFNPSSDVAALHKAIMVKGVDEATIIDILTKRNNAQRQQIKAAYLQETGKPLDETLKKALTGHLEEVVLALLKTPAQFDADELRAAMKGLGTDEDTLIEILASRTNKEIRDINRVYREELKRDLAKDITSDTSGDFRNALLSLAKGDRSEDFGVNEDLADSDARALYEAGERRKGTDVNVFNTILTTRSYPQLRRVFQKYTKYSKHDMNKVLDLELKGDIEKCLTAIVKCATSKPAFFAEKLHQAMKGVGTRHKALIRIMVSRSEIDMNDIKAFYQKMYGISLCQAILDETKGDYEKILVALCGGN

  • Molecular Weight

    Approximately 34-38 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 10% trehalose, 50 mM NaCl, 0.05% Tween 80, pH 7.5.
2.Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Zied Boudhraa,et al. Annexin A1 localization and its relevance to cancer. Clin Sci (Lond). 2016 Feb;130(4):205-20. [Content Brief]

[2]. Maurizio Bruschi, et al. Annexin A1 and Autoimmunity: From Basic Science to Clinical Applications. Int J Mol Sci. 2018 May 3;19(5):1348. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to inhibit proliferation of A549 human lung carcinoma cells. The ED50 for this effect is 0.7223 μg/mL, corresponding to a specific activity is 1.38×103 units/mg.

  • Annexin A1/ANXA1 Protein, Human Product Image
    1/16
  • Experimental Validation Results for Annexin A1/ANXA1 Protein, Human
  • Experimental Validation Results for Annexin A1/ANXA1 Protein, Human
  • Annexin A1/ANXA1 Protein, Human Product Image