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  5. TFRC Protein, Human (HEK293, His)

TFRC Protein, Human (HEK293, His)

Cat. No.: HY-P71069
SDS COA Handling Instructions

TFRC protein promotes cellular iron uptake through receptor-mediated endocytosis. TFRC is essential for erythroid and nervous system development and plays a crucial role in iron homeostasis. TFRC Protein, Human (HEK293, His) is the recombinant human-derived TFRC protein, expressed by HEK293 , with N-6*His labeled tag. The total length of TFRC Protein, Human (HEK293, His) is 660 a.a., with molecular weight of 78-90 kDa.

For research use only. We do not sell to patients.

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Description

TFRC protein promotes cellular iron uptake through receptor-mediated endocytosis. TFRC is essential for erythroid and nervous system development and plays a crucial role in iron homeostasis. TFRC Protein, Human (HEK293, His) is the recombinant human-derived TFRC protein, expressed by HEK293 , with N-6*His labeled tag. The total length of TFRC Protein, Human (HEK293, His) is 660 a.a., with molecular weight of 78-90 kDa.

Background

TFRC protein facilitates cellular iron uptake through receptor-mediated endocytosis of ligand-occupied transferrin receptors into specialized endosomes, as documented in studies. This process involves endosomal acidification, leading to iron release, followed by recycling of the apotransferrin-receptor complex to the cell surface, accompanied by a return to neutral pH and the subsequent loss of apotransferrin affinity for its receptor. Crucial for erythrocyte and nervous system development, TFRC is a vital player in iron homeostasis. The hereditary hemochromatosis protein HFE competes with transferrin for binding at an overlapping C-terminal site. TFRC positively regulates T and B cell proliferation through iron uptake and acts as a lipid sensor, modulating mitochondrial fusion by regulating the JNK pathway. Depending on dietary stearate levels, TFRC either promotes JNK pathway activation and degradation of the mitofusin MFN2 when stearate is low or inhibits JNK pathway activation and MFN2 degradation when stearate is high. Furthermore, TFRC acts as a receptor for new-world arenaviruses, including Guanarito, Junin, and Machupo virus, during microbial infection.

Biological Activity

Immobilized  Human Transferrin at 2 μg/mL (100 μL/well) can bind Biotinylated Human  Transferrin R that produces 50% of the optimal binding response is found to  be approximately 58.14 ng/mL.

Species

Human

Source

HEK293

Tag

N-6*His

Accession

P02786 (L101-F760)

Gene ID
Molecular Construction
N-term
6*His
TFRC (L101-F760)
Accession # P02786
C-term
Synonyms
Transferrin receptor protein 1; TR; TfR; Trfr; T9; p90
AA Sequence

LAGTESPVREEPGEDFPAARRLYWDDLKRKLSEKLDSTDFTGTIKLLNENSYVPREAGSQKDENLALYVENQFREFKLSKVWRDQHFVKIQVKDSAQNSVIIVDKNGRLVYLVENPGGYVAYSKAATVTGKLVHANFGTKKDFEDLYTPVNGSIVIVRAGKITFAEKVANAESLNAIGVLIYMDQTKFPIVNAELSFFGHAHLGTGDPYTPGFPSFNHTQFPPSRSSGLPNIPVQTISRAAAEKLFGNMEGDCPSDWKTDSTCRMVTSESKNVKLTVSNVLKEIKILNIFGVIKGFVEPDHYVVVGAQRDAWGPGAAKSGVGTALLLKLAQMFSDMVLKDGFQPSRSIIFASWSAGDFGSVGATEWLEGYLSSLHLKAFTYINLDKAVLGTSNFKVSASPLLYTLIEKTMQNVKHPVTGQFLYQDSNWASKVEKLTLDNAAFPFLAYSGIPAVSFCFCEDTDYPYLGTTMDTYKELIERIPELNKVARAAAEVAGQFVIKLTHDVELNLDYERYNSQLLSFVRDLNQYRADIKEMGLSLQWLYSARGDFFRATSRLTTDFGNAEKTDRFVMKKLNDRVMRVEYHFLSPYVSPKESPFRHVFWGSGSHTLPALLENLKLRKQNNGAFNETLFRNQLALATWTIQGAANALSGDVWDIDNEF

Molecular Weight

78-90 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 5% Trehalose, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Documentation

TFRC Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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TFRC Protein, Human (HEK293, His)
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HY-P71069
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