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TFRC Protein, Human (HEK293, His)

1 Cited Publications
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Based on 1 publication(s) in Google Scholar

TFRC protein promotes cellular iron uptake through receptor-mediated endocytosis. TFRC is essential for erythroid and nervous system development and plays a crucial role in iron homeostasis. TFRC Protein, Human (HEK293, His) is the recombinant human-derived TFRC protein, expressed by HEK293 , with N-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for TFRC Protein, Human (HEK293, His)
  • Experimental Validation Results for TFRC Protein, Human (HEK293, His)
  • Experimental Validation Results for TFRC Protein, Human (HEK293, His)
  • TFRC Protein, Human (HEK293, His) Product Image
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  • Biological Activity
  • Technical Parameters
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Biological Activity

Description

TFRC protein promotes cellular iron uptake through receptor-mediated endocytosis. TFRC is essential for erythroid and nervous system development and plays a crucial role in iron homeostasis. TFRC Protein, Human (HEK293, His) is the recombinant human-derived TFRC protein, expressed by HEK293 , with N-6*His labeled tag.

Background

TFRC protein facilitates cellular iron uptake through receptor-mediated endocytosis of ligand-occupied transferrin receptors into specialized endosomes, as documented in studies. This process involves endosomal acidification, leading to iron release, followed by recycling of the apotransferrin-receptor complex to the cell surface, accompanied by a return to neutral pH and the subsequent loss of apotransferrin affinity for its receptor. Crucial for erythrocyte and nervous system development, TFRC is a vital player in iron homeostasis. The hereditary hemochromatosis protein HFE competes with transferrin for binding at an overlapping C-terminal site. TFRC positively regulates T and B cell proliferation through iron uptake and acts as a lipid sensor, modulating mitochondrial fusion by regulating the JNK pathway. Depending on dietary stearate levels, TFRC either promotes JNK pathway activation and degradation of the mitofusin MFN2 when stearate is low or inhibits JNK pathway activation and MFN2 degradation when stearate is high. Furthermore, TFRC acts as a receptor for new-world arenaviruses, including Guanarito, Junin, and Machupo virus, during microbial infection.

Verified Bioactivity

Immobilized Human Transferrin at 2 μg/mL (100 μL/well) can bind Biotinylated Human Transferrin R that produces 50% of the optimal binding response is found to be approximately <59 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Immobilized Human Transferrin at 2 μg/mL (100 μL/well) can bind Biotinylated Human Transferrin R The ED50 for this effect is 6.822 ng/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag N-6*His
  • Accession

    P02786 (L101-F760)

  • Gene ID
    7037  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His
    • TFRC (L101-F760)
      Accession # P02786
    • C-term
  • Protein Length

    Full Length of Transferrin receptor protein 1, serum form Chain

  • Synonyms

    TFRC; Transferrin Receptor (P90, CD71); Transferrin Receptor; CD71 Antigen; P90; IMD46; Transferrin Receptor Protein 1; TRFR; CD71; Trfr; TFR1; TfR1; Transferrin Receptor 1; TFR; T9; TfR; TR

  • AA Sequence

    LAGTESPVREEPGEDFPAARRLYWDDLKRKLSEKLDSTDFTGTIKLLNENSYVPREAGSQKDENLALYVENQFREFKLSKVWRDQHFVKIQVKDSAQNSVIIVDKNGRLVYLVENPGGYVAYSKAATVTGKLVHANFGTKKDFEDLYTPVNGSIVIVRAGKITFAEKVANAESLNAIGVLIYMDQTKFPIVNAELSFFGHAHLGTGDPYTPGFPSFNHTQFPPSRSSGLPNIPVQTISRAAAEKLFGNMEGDCPSDWKTDSTCRMVTSESKNVKLTVSNVLKEIKILNIFGVIKGFVEPDHYVVVGAQRDAWGPGAAKSGVGTALLLKLAQMFSDMVLKDGFQPSRSIIFASWSAGDFGSVGATEWLEGYLSSLHLKAFTYINLDKAVLGTSNFKVSASPLLYTLIEKTMQNVKHPVTGQFLYQDSNWASKVEKLTLDNAAFPFLAYSGIPAVSFCFCEDTDYPYLGTTMDTYKELIERIPELNKVARAAAEVAGQFVIKLTHDVELNLDYERYNSQLLSFVRDLNQYRADIKEMGLSLQWLYSARGDFFRATSRLTTDFGNAEKTDRFVMKKLNDRVMRVEYHFLSPYVSPKESPFRHVFWGSGSHTLPALLENLKLRKQNNGAFNETLFRNQLALATWTIQGAANALSGDVWDIDNEF

  • Predicted Molecular Mass

    74.9 kDa

  • Molecular Weight

    Approximately 80-90 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, 5% trehalose, 5% mannitol, 0.01% Tween 80, pH 7.4.
3.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Immobilized Human Transferrin at 2 μg/mL (100 μL/well) can bind Biotinylated Human Transferrin R The ED50 for this effect is 6.822 ng/mL.

  • 1/16

    TFRC Protein, Human (HEK293, His) purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2025 Aug 30:776:152184. [Abstract]

    Customer Validation
     Binding capacity of 100 nM EGFP, 100 nM MAP30ER-EGFP to various recombinant proteins was measured by ELISA, with absorbance at 450 nm. Recombinant proteins (BSA, PD-1, EphA2, HGFR, TFRC (TFRC Protein, Human (HEK293, His)), HER3, L1CAM, LRP-1, HER2, Integrin alpha 5 beta 1, and EGFR, 1 ng/mL) were coated on 96-well plates overnight at 4 °C.

  • TFRC Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for TFRC Protein, Human (HEK293, His)
  • Experimental Validation Results for TFRC Protein, Human (HEK293, His)
  • Experimental Validation Results for TFRC Protein, Human (HEK293, His)
  • TFRC Protein, Human (HEK293, His) Product Image