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APLP-2 Protein, Human (HEK293, His)

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APLP-2, potentially involved in hemostasis regulation, exhibits inhibitory effects on coagulation factors. Its soluble form may interact with G-protein signaling pathways and bind to DNA. APLP-2 inhibits various proteases and modulates the nitric oxide-catalyzed autodegradation of GPC1 heparan sulfate side chains. It interacts with proteins like CPEB1, DAB2, APBB2/FE65L, and APBB3/FE65L2, suggesting diverse cellular roles. APLP-2 Protein, Human (HEK293, His) is the recombinant human-derived APLP-2 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • APLP-2 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

APLP-2, potentially involved in hemostasis regulation, exhibits inhibitory effects on coagulation factors. Its soluble form may interact with G-protein signaling pathways and bind to DNA. APLP-2 inhibits various proteases and modulates the nitric oxide-catalyzed autodegradation of GPC1 heparan sulfate side chains. It interacts with proteins like CPEB1, DAB2, APBB2/FE65L, and APBB3/FE65L2, suggesting diverse cellular roles. APLP-2 Protein, Human (HEK293, His) is the recombinant human-derived APLP-2 protein, expressed by HEK293 , with C-His labeled tag.

Background

APLP-2, a multifunctional protein, is implicated in the regulation of hemostasis, with its soluble form demonstrating inhibitory properties towards coagulation factors. This protein may engage with cellular G-protein signaling pathways and exhibits binding to the DNA 5'-GTCACATG-3' (CDEI box). APLP-2 showcases diverse inhibitory activities, including trypsin, chymotrypsin, plasmin, factor XIA, and plasma and glandular kallikrein. Moreover, it modulates the Cu/Zn nitric oxide-catalyzed autodegradation of GPC1 heparan sulfate side chains in fibroblasts. In its molecular interactions, APLP-2 engages with CPEB1 and interacts with DAB2 via its NPXY motif, with this interaction being influenced by tyrosine phosphorylation. Additionally, APLP-2 associates with APBB2/FE65L and APBB3/FE65L2, underscoring its involvement in intricate cellular networks.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-8*His
  • Accession

    Q06481-1 (G32-S692)

  • Gene ID
    334  [NCBI]
  • Molecular Construction
    • N-term
    • APLP-2 (G32-S692)
      Accession # Q06481-1
    • 8*His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    APLP2; Amyloid Protein Homolog; Prev. APPL2; APLP-2; Amyloid Beta (A4) Precursor-Like Protein 2; CDEBP; APPH; Amyloid Precursor Protein Homolog HSD-2; Sperm Membrane Protein YWK-II; Testicular Tissue Protein Li 23; CDEI Box-Binding Protein; Alternative Pr

  • AA Sequence

    GYIEALAANAGTGFAVAEPQIAMFCGKLNMHVNIQTGKWEPDPTGTKSCFETKEEVLQYCQEMYPELQITNVMEANQRVSIDNWCRRDKKQCKSRFVTPFKCLVGEFVSDVLLVPEKCQFFHKERMEVCENHQHWHTVVKEACLTQGMTLYSYGMLLPCGVDQFHGTEYVCCPQTKIIGSVSKEEEEEDEEEEEEEDEEEDYDVYKSEFPTEADLEDFTEAAVDEDDEDEEEGEEVVEDRDYYYDTFKGDDYNEENPTEPGSDGTMSDKEITHDVKAVCSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESEDYCMAVCKAMIPPTPLPTNDVDVYFETSADDNEHARFQKAKEQLEIRHRNRMDRVKKEWEEAELQAKNLPKAERQTLIQHFQAMVKALEKEAASEKQQLVETHLARVEAMLNDRRRMALENYLAALQSDPPRPHRILQALRRYVRAENKDRLHTIRHYQHVLAVDPEKAAQMKSQVMTHLHVIEERRNQSLSLLYKVPYVAQEIQEEIDELLQEQRADMDQFTASISETPVDVRVSSEESEEIPPFHPFHPFPALPENEDTQPELYHPMKKGSGVGEQDGGLIGAEEKVINSKNKVDENMVIDETLDVKEMIFNAERVGGLEEERESVGPLREDFSLSS

  • Predicted Molecular Mass

    77.2 kDa

  • Molecular Weight

    Approximately 80-100 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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