ApoA-IV Protein, Mouse (P.pastoris, His)

ApoA-IV Protein plays a vital role in lipid metabolism, influencing chylomicron and VLDL secretion and catabolism. It efficiently activates lipoprotein lipase with ApoC-II and serves as a potent activator of LCAT. As a component of HDL and chylomicrons, ApoA-IV is integral to cholesterol transport and metabolism. Structurally, the protein forms homodimers, reflecting its crucial role in lipid-related processes. ApoA-IV Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived ApoA-IV protein, expressed by P. pastoris , with N-6*His labeled tag.

The ApoA-IV protein is implicated in various aspects of lipid metabolism, playing a potential role in the secretion and catabolism of chylomicrons and very-low-density lipoproteins (VLDL). It is essential for the efficient activation of lipoprotein lipase by ApoC-II and serves as a potent activator of lecithin-cholesterol acyltransferase (LCAT). ApoA-IV emerges as a significant component of high-density lipoproteins (HDL) and chylomicrons, highlighting its involvement in both cholesterol transport and metabolism. Structurally, the protein forms homodimers, indicative of its molecular arrangement in these crucial lipid-related processes.

Mouse

P. pastoris

N-6*His

P06728 (E21-S395)

11808  [NCBI]

Full Length of Mature Protein

EVTSDQVANVVWDYFTQLSNNAKEAVEQFQKTDVTQQLSTLFQDKLGDASTYADGVHNKLVPFVVQLSGHLAQETERVKEEIKKELEDLRDRMMPHANKVTQTFGENMQKLQEHLKPYAVDLQDQINTQTQEMKLQLTPYIQRMQTTIKENVDNLHTSMMPLATNLKDKFNRNMEELKGHLTPRANELKATIDQNLEDLRRSLAPLTVGVQEKLNHQMEGLAFQMKKNAEELQTKVSAKIDQLQKNLAPLVEDVQSKVKGNTEGLQKSLEDLNRQLEQQVEEFRRTVEPMGEMFNKALVQQLEQFRQQLGPNSGEVESHLSFLEKSLREKVNSFMSTLEKKGSPDQPQALPLPEQAQEQAQEQAQEQVQPKPLES

Approximately 45.0 kDa

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.