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Apolipoprotein H/APOH Protein, Mouse (HEK293, His)

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Based on 1 Customer Validation

Apolipoprotein H/APOH protein binds to heparin, phospholipids, and dextran sulfate, preventing activation of the blood coagulation cascade by interacting with phospholipids on damaged cell surfaces. Apolipoprotein H/APOH Protein, Mouse (HEK293, His) is the recombinant mouse-derived Apolipoprotein H/APOH protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Apolipoprotein H/APOH Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Apolipoprotein H/APOH Protein, Mouse (HEK293, His)
  • Apolipoprotein H/APOH Protein, Mouse (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

Apolipoprotein H/APOH protein binds to heparin, phospholipids, and dextran sulfate, preventing activation of the blood coagulation cascade by interacting with phospholipids on damaged cell surfaces. Apolipoprotein H/APOH Protein, Mouse (HEK293, His) is the recombinant mouse-derived Apolipoprotein H/APOH protein, expressed by HEK293 , with C-His labeled tag.

Background

Apolipoprotein H/APOH protein is known for its ability to bind to negatively charged substances including heparin, phospholipids, and dextran sulfate. It is believed to play a role in preventing the activation of the intrinsic blood coagulation cascade by interacting with phospholipids found on the surface of damaged cells.

Verified Bioactivity

Measured in a cell proliferation assay using Jurkat human T-lymphocyte leukemia cells. The ED50 this effect is 0.9606 μg/mL, corresponding to a specific activity is 1.041×103 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured in a cell proliferation assay using Jurkat human T-lymphocyte leukemia cells. The ED50 this effect is 0.9606 μg/mL, corresponding to a specific activity is 1.041×103 units/mg.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-His
  • Accession

    Q01339 (G20-C345)

  • Gene ID
    11818  [NCBI]
  • Molecular Construction
    • N-term
    • APOH (G20-C345)
      Accession # Q01339
    • His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    APOH; Anticardiolipin Cofactor; Prev. B2G1; Beta(2)GPI; Beta-2-Glycoprotein I; B2GPI; Beta-2-Glycoprotein 1; Apo-H; BG; Epididymis Secretory Sperm Binding Protein; Apolipoprotein H (Beta-2-Glycoprotein I); B2GP1; Activated Protein C-Binding Protein; Apoli

  • AA Sequence

    GRICPKPDDLPFATVVPLKTSYDPGEQIVYSCKPGYVSRGGMRRFTCPLTGMWPINTLRCVPRVCPFAGILENGIVRYTSFEYPKNISFACNPGFFLNGTSSSKCTEEGKWSPDIPACARITCPPPPVPKFALLKDYRPSAGNNSLYQDTVVFKCLPHFAMIGNDTVMCTEQGNWTRLPECLEVKCPFPPRPENGYVNYPAKPVLLYKDKATFGCHETYKLDGPEEAECTKTGTWSFLPTCRESCKLPVKKATVLYQGMRVKIQEQFKNGMMHGDKIHFYCKNKEKKCSYTVEAHCRDGTIEIPSCFKEHSSLAFWKTDASELTPC

  • Molecular Weight

    Approximately 50-62 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl or PBS, pH 7.4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Mass (in vial) Mass (in vial)
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Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured in a cell proliferation assay using Jurkat human T-lymphocyte leukemia cells. The ED50 this effect is 0.9606 μg/mL, corresponding to a specific activity is 1.041×103 units/mg.

  • Apolipoprotein H/APOH Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Apolipoprotein H/APOH Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Apolipoprotein H/APOH Protein, Mouse (HEK293, His)
  • Apolipoprotein H/APOH Protein, Mouse (HEK293, His) Product Image