Arylsulfatase A/ARSA Protein, Mouse (HEK293, His)

Arylsulfatase A (ARSA) possesses arylsulfatase and calcium ion binding activities. It plays a critical role in sperm-zona pellucida binding and is located in the plasma membrane. ARSA is expressed in the brain and genitourinary system, reflecting its involvement in various processes. It is associated with metachromatic leukodystrophy and broadly expressed across tissues, highlighting its significance in diverse contexts. Arylsulfatase A/ARSA Protein, Mouse (HEK293, His) is the recombinant mouse-derived Arylsulfatase A/ARSA protein, expressed by HEK293 , with C-10*His labeled tag. The total length of Arylsulfatase A/ARSA Protein, Mouse (HEK293, His) is 489 a.a., with molecular weight of ~65 kDa.

Arylsulfatase A (ARSA) is predicted to possess arylsulfatase activity and calcium ion binding activity. It plays a critical role upstream of the binding activity of sperm to the zona pellucida and is located in the plasma membrane, serving as an integral component of the membrane. ARSA is expressed in the brain and genitourinary system, reflecting its involvement in various physiological processes. This gene is associated with metachromatic leukodystrophy, a condition characterized by the abnormal accumulation of sulfatides in the central and peripheral nervous systems. The broad expression of ARSA across tissues, including the testis, lung, and numerous other organs, underscores its significance in diverse biological contexts.

Measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS). The specific activity is 9.387×10^4 pmoL/min/µg, as measured under the described conditions.

Mouse

HEK293

C-10*His

NP_033843.2 (S18-S506)

11883  [NCBI]

SPPNILLIFADDLGYGDLGSYGHPSSTTPNLDQLAEGGLRFTDFYVPVSLCTPSRAALLTGRLPVRSGMYPGVLGPSSQGGLPLEEVTLAEVLAARGYLTGMAGKWHLGVGPEGAFLPPHQGFHRFLGIPYSHDQGPCQNLTCFPPDIPCKGGCDQGLVPIPLLANLTVEAQPPWLPGLEARYVSFSRDLMADAQRQGRPFFLYYASHHTHYPQFSGQSFTKRSGRGPFGDSLMELDGAVGALMTTVGDLGLLEETLVIFTADNGPELMRMSNGGCSGLLRCGKGTTFEGGVREPALVYWPGHITPGVTHELASSLDLLPTLAALTGAPLPNVTLDGVDISPLLLGTGKSPRKSVFFYPPYPDEIHGVFAVRNGKYKAHFFTQGSAHSDTTSDPACHAANRLTAHEPPLLYDLSQDPGENYNVLESIEGVSPEALQALKHIQLLKAQYDAAMTFGPSQIAKGEDPALQICCQPSCTPHPVCCHCPGSQS

Approximately 65 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.