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NPR2 Protein, Human (P. pastoris, His)

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Based on 1 publication(s) in Google Scholar

NPR2 Protein, Human (P. pastoris, His) serves as the receptor for C-type natriuretic peptide NPPC/CNP, activating guanylate cyclase upon ligand binding. NPR2 potentially regulates skeletal growth, indicating involvement in bone development. Its interaction with C-type natriuretic peptide highlights its significance in mediating cellular responses, especially in signaling pathways influencing skeletal growth dynamics. NPR2 Protein, Human (P. pastoris, His) is the recombinant human-derived Atrial natriuretic peptide receptor 2/NPR2, expressed by P. pastoris , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: P. pastoris
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for NPR2 Protein, Human (P. pastoris, His)
  • NPR2 Protein, Human (P. pastoris, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

NPR2 Protein, Human (P. pastoris, His) serves as the receptor for C-type natriuretic peptide NPPC/CNP, activating guanylate cyclase upon ligand binding. NPR2 potentially regulates skeletal growth, indicating involvement in bone development. Its interaction with C-type natriuretic peptide highlights its significance in mediating cellular responses, especially in signaling pathways influencing skeletal growth dynamics. NPR2 Protein, Human (P. pastoris, His) is the recombinant human-derived Atrial natriuretic peptide receptor 2/NPR2, expressed by P. pastoris , with N-His labeled tag.

Background

Atrial natriuretic peptide receptor 2 (NPR2) functions as a receptor for the C-type natriuretic peptide NPPC/CNP hormone, exhibiting guanylate cyclase activity upon ligand binding. This receptor plays a potential role in the regulation of skeletal growth, suggesting its involvement in processes that govern bone development and maintenance. The interaction between NPR2 and C-type natriuretic peptide highlights its significance in mediating cellular responses to this hormone, particularly in the context of signaling pathways that influence skeletal growth dynamics.

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source P. pastoris
  • Tag N-His
  • Accession

    P20594-1 (R23-I458)

  • Gene ID
    4882  [NCBI]
  • Molecular Construction
    • N-term
    • His
    • ANPRB (R23-I458)
      Accession # P20594-1
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    NPR2; Atrial Natriuretic Peptide B-Type Receptor; Prev. ANPRB; Atrionatriuretic Peptide Receptor B; Prev. AMDM; Natriuretic Peptide Receptor B; Prev. NPRB; Guanylate Cyclase; GC-B; ANPR-B; Atrial Natriuretic Peptide Receptor Type B; GUC2B; Atrial Natriure

  • AA Sequence

    RNLTLAVVLPEHNLSYAWAWPRVGPAVALAVEALGRALPVDLRFVSSELEGACSEYLAPLSAVDLKLYHDPDLLLGPGCVYPAASVARFASHWRLPLLTAGAVASGFSAKNDHYRTLVRTGPSAPKLGEFVVTLHGHFNWTARAALLYLDARTDDRPHYFTIEGVFEALQGSNLSVQHQVYAREPGGPEQATHFIRANGRIVYICGPLEMLHEILLQAQRENLTNGDYVFFYLDVFGESLRAGPTRATGRPWQDNRTREQAQALREAFQTVLVITYREPPNPEYQEFQNRLLIRAREDFGVELGPSLMNLIAGCFYDGILLYAEVLNETIQEGGTREDGLRIVEKMQGRRYHGVTGLVVMDKNNDRETDFVLWAMGDLDSGDFQPAAHYSGAEKQIWWTGRPIPWVKGAPPSDNPPCAFDLDDPSCDKTPLSTLAI

  • Predicted Molecular Mass

    50 kDa

  • Molecular Weight

    Approximately 51 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
3.Lyophilized from a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1.0 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • NPR2 Protein, Human (P. pastoris, His) Product Image
    1/16
  • Experimental Validation Results for NPR2 Protein, Human (P. pastoris, His)
  • NPR2 Protein, Human (P. pastoris, His) Product Image