B2M/Beta-2-microglobulin Protein, Human (99a.a, HEK293, His)

B2M is a component of MHC class I complexes that present peptide antigens to the immune system. B2M/Beta-2-microglobulin Protein, Human (99a.a, HEK293, His) is the recombinant human-derived B2M/Beta-2-microglobulin protein, expressed by HEK293 , with C-6*His labeled tag. The total length of B2M/Beta-2-microglobulin Protein, Human (99a.a, HEK293, His) is 99 a.a., with molecular weight of ~14.0 kDa.

B2M, or Beta-2-microglobulin, functions as a critical component of the class I major histocompatibility complex (MHC), playing a central role in presenting peptide antigens to the immune system. Notably, exogenously applied M. tuberculosis EsxA or EsxA-EsxB binds B2M and reduces its export to the cell surface, potentially leading to defects in class I antigen presentation. B2M exists as a heterodimer, composed of an alpha chain and a beta chain, with the latter serving as the beta-chain of major histocompatibility complex class I molecules. Polymers of B2M have been observed in tissues of patients on long-term hemodialysis. B2M, in its isolated form, interacts with M. tuberculosis EsxA and an EsxA-EsxB complex, forming a tripartite complex detectable in the host endoplasmic reticulum. The stability of the B2M-EsxA complex extends across a broad pH range and in the presence of high salt concentrations. Additionally, B2M forms heterotrimers with HLA-E, HLA-G, and HLA-F, along with a self- or foreign peptide, contributing to the diverse functions of the major histocompatibility complex. Furthermore, B2M engages in a heterotrimeric complex with MR1, playing a role in antigen presentation associated with metabolite antigens.

Human

HEK293

C-6*His

P61769 (I21-M119)

567  [NCBI]

IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM

Approximately 14.0 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.