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  4. B7-1/CD80
  5. B7-1/CD80 Protein, Cynomolgus (208a.a, HEK293, His)

B7-1/CD80 Protein, Cynomolgus (208a.a, HEK293, His)

Cat. No.: HY-P72767
COA Handling Instructions

CD80 is a costimulatory cytokine for cancer immunity that is activated by binding to CD28 or CTLA-4. Tumor immune evasion occurs when CD80 expression is low. The increased expression of CD80 induced by TP53 stimulates anti-tumor immune responses. B7-1/CD80 Protein, Cynomolgus (208a.a, HEK293, His) is the recombinant cynomolgus-derived B7-1/CD80 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of B7-1/CD80 Protein, Cynomolgus (208a.a, HEK293, His) is 208 a.a., with molecular weight of 35-55 kDa.

For research use only. We do not sell to patients.

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Description

CD80 is a costimulatory cytokine for cancer immunity that is activated by binding to CD28 or CTLA-4. Tumor immune evasion occurs when CD80 expression is low. The increased expression of CD80 induced by TP53 stimulates anti-tumor immune responses. B7-1/CD80 Protein, Cynomolgus (208a.a, HEK293, His) is the recombinant cynomolgus-derived B7-1/CD80 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of B7-1/CD80 Protein, Cynomolgus (208a.a, HEK293, His) is 208 a.a., with molecular weight of 35-55 kDa.

Background

CD80 is a membrane receptor and costimulatory cytokine used by immune cells to limit cancer progression. CD80 is activated by binding to CD28 or CTLA-4, thereby inducing T cell proliferation and cytokine production. The immune evasion mechanism of colon cancer is related to the low surface expression of CD80. When CD80 is upregulated on the surface of tumor cells, the anti-tumor immune response can be successfully activated. Normal expression levels of CD80 are frequently lost during tumor progression, possibly due to selective pressure from the immune system. TP53 is a regulator of CD80 expression in human cancer cells, and CD80 expression is strongly correlated with TP53 activation. TP53 induces antitumor immune responses through induction of CD80 in human cancer epithelial cells. CD80 serves as a receptor for adenovirus subtype B and may play a role in lupus neuropathy. CD80/CD86-conjugated adenovirus serotype 5 (Ad5)/3 chimeras improve expression and transduction efficiency with excellent transduction efficiency and low toxicity in brain tumors. Additionally, measurement of urinary CD80 excretion levels was able to differentiate between minimal change disease and focal segmental glomerulosclerosis in a mouse model of foot process effacement and human lupus nephritis.

Biological Activity

Loaded Cynomolgus CTLA-4-Fc on Protein A Biosensor, can bind Cynomolgus B7-1-His with an affinity constant of 0.87 uM as determined in BLI assay.

Species

Cynomolgus

Source

HEK293

Tag

C-6*His

Accession

G7NXN7 (V35-N242)

Gene ID

102140078  [NCBI]

Molecular Construction
N-term
B7-1 (V35-N242)
Accession # G7NXN7
6*His
C-term
Synonyms
T-lymphocyte activation antigen CD80; Activation B7-1 antigen; B7; CD80
AA Sequence

VATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVMLSVKADFPTPSITDFEIPPSNIRRIICSTSGGFPEPHLSWLENGEELNAISTTVSQDPETELYTVSSKLDFNMTTNHSFMCLIKYGHLRVNQTFNWNTPKQEHFPDN

Molecular Weight

35-55 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
B7-1/CD80 Protein, Cynomolgus (208a.a, HEK293, His)
Cat. No.:
HY-P72767
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