B7-H4 Protein, Human (Biotinylated, HEK293, His-Avi)
Based on 1 Customer Validation
The B7-H4 protein acts as a negative regulator, inhibiting T cell-mediated immune responses, including activation, proliferation, cytokine production, and cytotoxicity. When expressed on tumor macrophages, it cooperates with regulatory T cells to suppress tumor-associated antigen-specific T cell immunity. B7-H4 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived B7-H4 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- Species: Human
- Source: HEK293
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保管条件:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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生物活性
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Technical Parameters
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Product Properties
- ドキュメント
- Help & FAQs
生物活性
The B7-H4 protein acts as a negative regulator, inhibiting T cell-mediated immune responses, including activation, proliferation, cytokine production, and cytotoxicity. When expressed on tumor macrophages, it cooperates with regulatory T cells to suppress tumor-associated antigen-specific T cell immunity. B7-H4 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived B7-H4 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.
B7-H4 protein functions as a negative regulator of the T-cell-mediated immune response, exerting inhibitory effects on T-cell activation, proliferation, cytokine production, and the development of cytotoxicity. Particularly significant when expressed on the cell surface of tumor macrophages, B7-H4, in collaboration with regulatory T-cells (Treg), plays a crucial role in suppressing tumor-associated antigen-specific T-cell immunity. Additionally, B7-H4 is implicated in the promotion of epithelial cell transformation, indicating its multifaceted involvement in modulating immune responses and cellular processes associated with tumor development.
1. Measured by its binding ability in a functional ELISA. When immobilized Anti-B7-H4 Antibody at 1μg/ml (100μl/well), can bind Biotinylated Human B7-H4, His Tag and the EC50 is < 65 ng/mL.
2. Loaded Puxitatug (HY-P990059) on AHC2 biosensor, can bind B7-H4 Protein, Human (Biotinylated, HEK293, His-Avi) with an affinity constant of 4.217E-09 M as determined in BLI assay.
3. Loaded Emiltatug (HY-P990918) on AHC2 biosensor, can bind B7-H4 Protein, Human (Biotinylated, HEK293, His-Avi) with an affinity constant of 8.115E-09 M as determined in BLI assay.
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Loaded Puxitatug (HY-P990059) on AHC2 biosensor, can bind B7-H4 Protein, Human (Biotinylated, HEK293, His-Avi) with an affinity constant of 4.217E-09 M as determined in BLI assay. -
Loaded Emiltatug (HY-P990918) on AHC2 biosensor, can bind B7-H4 Protein, Human (Biotinylated, HEK293, His-Avi) with an affinity constant of 8.115E-09 M as determined in BLI assay.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-Avi;C-8*His
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アクセッション番号
Q7Z7D3-1 (F29-A258)
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Molecular Construction
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N-term
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B7-H4 (F29-A258)
Accession # Q7Z7D3-1 -
8*His-Avi
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C-term
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Protein Length
Extracellular Domain
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別名
B7H4; B7-H4; B7S1; B7h.5; B7x; FLJ22418; VTCN1; PRO1291
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AA Sequence
FGISGRHSITVTTVASAGNIGEDGILSCTFEPDIKLSDIVIQWLKEGVLGLVHEFKEGKDELSEQDEMFRGRTAVFADQVIVGNASLRLKNVQLTDAGTYKCYIITSKGKGNANLEYKTGAFSMPEVNVDYNASSETLRCEAPRWFPQPTVVWASQVDQGANFSEVSNTSFELNSENVTMKVVSVLYNVTINNTYSCMIENDIAKATGDIKVTESEIKRRSHLQLLNSKA
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Predicted Molecular Mass
28.2 kDa
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分子量
Approximately 60-70 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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純度
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
ドキュメント
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データシート (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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取扱説明書 (2659 KB)
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
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