BCCIP Protein, Human (sf9)
BCCIP proteins contribute to interphase microtubule organization and anchoring as well as spindle pole organization and stability during mitosis. BCCIP protein may induce cell cycle arrest by enhancing CDK2 inhibition and participate in DNA damage repair through homologous recombination with BRCA2. BCCIP Protein, Human (sf9) is the recombinant human-derived BCCIP protein, expressed by Sf9 insect cells, with tag free.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
BCCIP proteins contribute to interphase microtubule organization and anchoring as well as spindle pole organization and stability during mitosis. BCCIP protein may induce cell cycle arrest by enhancing CDK2 inhibition and participate in DNA damage repair through homologous recombination with BRCA2. BCCIP Protein, Human (sf9) is the recombinant human-derived BCCIP protein, expressed by Sf9 insect cells, with tag free.
Background
BCCIP, a multifaceted protein, plays crucial roles during interphase and mitosis. In interphase, it is essential for microtubule organizing and anchoring activities, while during mitosis, it contributes significantly to spindle pole organization and stabilization. Notably, isoform 2/alpha emerges as particularly vital in regulating microtubule anchoring, stability, spindle architecture, and orientation, compared to isoform 1/beta. Beyond its structural functions, BCCIP may exert control over the cell cycle by enhancing CDKN1A-mediated inhibition of CDK2 activity, potentially promoting cell cycle arrest. Furthermore, BCCIP may play a role in DNA damage repair through homologous recombination, likely in conjunction with BRCA2, while its involvement in non-homologous end joining (NHEJ) remains unclear. BCCIP engages in diverse interactions, forming complexes with BRCA2, CDKN1A, MTDH/LYRIC, DCTN1/p150-glued, ACTR1A/ARP1, and tubulin subunits. Isoforms 1 and 2 exhibit distinct interaction profiles, with both associating with alpha-, beta-, and gamma-tubulins. Additionally, BCCIP interacts with TENT5C, although this interaction does not impact TENT5C poly(A) polymerase function.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag Tag Free
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Accession
Q9P287-1 (N-G&P, M1-V314)
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Molecular Construction
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N-term
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Gly-Pro
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BCCIP (M1-V314)
Accession # Q9P287-1 -
C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
BCCIP; TOK1; BRCA2 And CDKN1A Interacting Protein; BRCA2 And Cip1/P21 Interacting Protein; BRCA2 And CDKN1A-Interacting Protein; Cdk Inhibitor P21 Binding Protein; BCCIPalpha; TOK-1alpha; TOK-1; BCCIPbeta; P21- And CDK-Associated Protein 1; TOK-1beta; Pro
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Predicted Molecular Mass
36.2 kDa
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Molecular Weight
Approximately 47 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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