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Bcl-2-like protein 2 Protein, Human (His)

Cat. No.: HY-P7653
COA Handling Instructions

Bcl-2-like protein 2 is a key regulator that promotes cell survival and prevents dexamethasone-induced apoptosis, which is critical for postmitotic Sertoli cells. It inhibits BAX, emphasizing its role in survival mechanisms. Bcl-2-like protein 2 Protein, Human (His) is the recombinant human-derived Bcl-2-like protein 2 protein, expressed by E. coli , with C-6*His labeled tag. The total length of Bcl-2-like protein 2 Protein, Human (His) is 171 a.a., with molecular weight of ~18.0 kDa.

For research use only. We do not sell to patients.

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5 μg $35 In-stock
10 μg $60 In-stock
50 μg $165 In-stock
100 μg $280 In-stock
500 μg $785 In-stock
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Description

Bcl-2-like protein 2 is a key regulator that promotes cell survival and prevents dexamethasone-induced apoptosis, which is critical for postmitotic Sertoli cells. It inhibits BAX, emphasizing its role in survival mechanisms. Bcl-2-like protein 2 Protein, Human (His) is the recombinant human-derived Bcl-2-like protein 2 protein, expressed by E. coli , with C-6*His labeled tag. The total length of Bcl-2-like protein 2 Protein, Human (His) is 171 a.a., with molecular weight of ~18.0 kDa.

Background

Bcl-2-like protein 2, a crucial player in cellular dynamics, exerts its influence by promoting cell survival and effectively blocking dexamethasone-induced apoptosis. This multifunctional protein plays a pivotal role in the survival of postmitotic Sertoli cells, where it suppresses the death-promoting activity of BAX. The intricate molecular network extends to interactions with HIF3A through its C-terminus domain, emphasizing its involvement in complex signaling pathways. Additionally, Bcl-2-like protein 2 engages in interactions with BOP, further highlighting its role in cellular homeostasis. The nuanced functions of Bcl-2-like protein 2 underscore its significance in orchestrating cell survival mechanisms and warrant further exploration to comprehensively understand its molecular contributions in diverse cellular contexts.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized BID at 5 μg/mL (100 μL/well) can bind human Bcl-2-like protein 2. The ED50 for this effect is 10.09 ng/mL.

  • Measured by its binding ability in a functional ELISA. Immobilized BID at 5 μg/mL (100 μL/well) can bind human Bcl-2-like protein 2. The ED50 for this effect is 10.09 ng/mL.
Species

Human

Source

E. coli

Tag

C-6*His

Accession

AAI13523.1 (A2-T172)

Gene ID

599  [NCBI]

Molecular Construction
N-term
BCL2L2 (A2-T172)
Accession # Q92843
6*His
C-term
Synonyms
rHuBcl-2-like protein 2, His; Bcl-2-Like Protein 2; Apoptosis Regulator Bcl-W; BCL2L2; KIAA0271
AA Sequence

ATPASAPDTRALVADFVGYKLRQKGYVCGAGPGEGPAADPLHQAMRAAGDEFETRFRRTFSDLAAQLHVTPGSAQQRFTQVSDELFQGGPNWGRLVAFFVFGAALCAESVNKEMEPLVGQVQEWMVAYLETRLADWIHSSGGWAEFTALYGDGALEEARRLREGNWASVRT

Molecular Weight

Approximately 18.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Solution

Formulation

Supplied as a 0.2 μm filter solution of 25 mM HEPES, 100 mM KCl, 20% Glycerol, pH 7.5 or 20 mM HEPES, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation
References

Bcl-2-like protein 2 Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Bcl-2-like protein 2 Protein, Human (His)
Cat. No.:
HY-P7653
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