BLMH/Bleomycin Hydrolase Protein, Mouse (P. pastoris, His)
Bleomycin Hydrolase Protein (BLMH) is crucial for cellular defense, inactivating the antitumor drug Bleomycin (BLM) by hydrolyzing its carboxamide bond. This neutralizes BLM's cytotoxic effects, protecting normal and malignant cells. BLMH's exact physiological function is unknown, but its significance in maintaining cellular balance and defending against harmful agents is evident. BLMH/Bleomycin Hydrolase Protein, Mouse (P. pastoris, His) is the recombinant mouse-derived BLMH/Bleomycin Hydrolase Protein, expressed by P. pastoris , with N-6*His labeled tag.
- Species: Mouse
- Source: P. pastoris
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
Bleomycin Hydrolase Protein (BLMH) is crucial for cellular defense, inactivating the antitumor drug Bleomycin (BLM) by hydrolyzing its carboxamide bond. This neutralizes BLM's cytotoxic effects, protecting normal and malignant cells. BLMH's exact physiological function is unknown, but its significance in maintaining cellular balance and defending against harmful agents is evident. BLMH/Bleomycin Hydrolase Protein, Mouse (P. pastoris, His) is the recombinant mouse-derived BLMH/Bleomycin Hydrolase Protein, expressed by P. pastoris , with N-6*His labeled tag.
Background
BLM hydrolase, also known as Bleomycin hydrolase, plays a crucial role in cellular defense mechanisms by catalyzing the inactivation of the antitumor drug Bleomycin (BLM), a glycopeptide. This enzymatic activity involves the hydrolysis of the carboxamide bond within the B-aminoalaninamide moiety of BLM, leading to its inactivation. This process serves to protect both normal and malignant cells from the toxic effects of BLM. While the precise physiological function of BLM hydrolase remains unclear, its ability to neutralize the cytotoxic impact of BLM highlights its significance in cellular homeostasis and defense against potentially harmful agents.
Technical Parameters
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Species Mouse
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Source P. pastoris
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Tag N-6*His
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Accession
Q8R016 (M1-E455)
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Molecular Construction
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N-term
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6*His
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BLMH (M1-E455)
Accession # Q8R016 -
C-term
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Protein Length
Full Length
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Synonyms
BLMH; BLM Hydrolase; Bleomycin Hydrolase; BMH; BH
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AA Sequence
MNNAGLNSEKVSALIQKLNSDPQFVLAQNVGTTHDLLDICLRRATVQGAQHVFQHVVPQEGKPVTNQKSSGRCWIFSCLNVMRLPFMKKFNIEEFEFSQSYLFFWDKVERCYFFLNAFVDTAQKKEPEDGRLVQYLLMNPTNDGGQWDMLVNIVEKYGVVPKKCFPESHTTEATRRMNDILNHKMREFCIRLRNLVHSGATKGEISSTQDAMMEEIFRVVCICLGNPPETFTWEYRDKDKNYHKIGPITPLQFYKEHVKPLFNMEDKICFVNDPRPQHKYNKLYTVDYLSNMVGGRKTLYNNQPIDFLKKMVAASIKDGEAVWFGCDVGKHFNGKLGLSDMNVYDHELVFGVSLKNMNKAERLAFGESLMTHAMTFTAVSEKDNQEGTFVKWRVENSWGEDHGHKGYLCMTDEWFSEYVYEVVVDKKHVPEEVLAVLEQEPIVLPAWDPMGALAE
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Predicted Molecular Mass
53.8 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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