C1s/Complement component C1s Protein, Human (HEK293, His)
Based on 1 Customer Validation
C1s/Complement component C1s, a serine protease and B chain, forms C1 with C1q and C1r, initiating the classical complement pathway. Activated by C1r, C1s then activates downstream components C2 and C4, crucially driving the complement system cascade. C1s/Complement component C1s Protein, Human (HEK293, His) is the recombinant human-derived C1s/Complement component C1s protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
C1s/Complement component C1s, a serine protease and B chain, forms C1 with C1q and C1r, initiating the classical complement pathway. Activated by C1r, C1s then activates downstream components C2 and C4, crucially driving the complement system cascade. C1s/Complement component C1s Protein, Human (HEK293, His) is the recombinant human-derived C1s/Complement component C1s protein, expressed by HEK293 , with C-His labeled tag.
C1s, a serine protease, constitutes the B chain that, in conjunction with C1q and C1r, assembles to create C1, the initial component in the classical pathway of the complement system. Activation of C1s is facilitated by C1r, enabling it to subsequently activate downstream components C2 and C4, thereby initiating the cascade of events essential for complement system function.
1.Immobilized Human Complement C1s, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-C1S Antibody, hFc Tag with the EC50 of 2.9-5.0 ng/mL determined by ELISA.
2. Loaded Claseprubart (HY-P991127) on ProA biosensor, can bind C1s/Complement C1s Protein, Human (HEK293, His) with an affinity constant of 3.648E-09 M as determined in BLI assay.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-8*His
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Accession
P09871 (E16-D688)
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Molecular Construction
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N-term
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C1s (E16-D688)
Accession # P09871 -
8*His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
C1S; Complement C1s Subcomponent; Complement C1s; C1 Esterase; Complement Component 1, S Subcomponent; Basic Proline-Rich Peptide IB-1; Complement Component 1 Subcomponent S; EDSPD2
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AA Sequence
EPTMYGEILSPNYPQAYPSEVEKSWDIEVPEGYGIHLYFTHLDIELSENCAYDSVQIISGDTEEGRLCGQRSSNNPHSPIVEEFQVPYNKLQVIFKSDFSNEERFTGFAAYYVATDINECTDFVDVPCSHFCNNFIGGYFCSCPPEYFLHDDMKNCGVNCSGDVFTALIGEIASPNYPKPYPENSRCEYQIRLEKGFQVVVTLRREDFDVEAADSAGNCLDSLVFVAGDRQFGPYCGHGFPGPLNIETKSNALDIIFQTDLTGQKKGWKLRYHGDPMPCPKEDTPNSVWEPAKAKYVFRDVVQITCLDGFEVVEGRVGATSFYSTCQSNGKWSNSKLKCQPVDCGIPESIENGKVEDPESTLFGSVIRYTCEEPYYYMENGGGGEYHCAGNGSWVNEVLGPELPKCVPVCGVPREPFEEKQRIIGGSDADIKNFPWQVFFDNPWAGGALINEYWVLTAAHVVEGNREPTMYVGSTSVQTSRLAKSKMLTPEHVFIHPGWKLLEVPEGRTNFDNDIALVRLKDPVKMGPTVSPICLPGTSSDYNLMDGDLGLISGWGRTEKRDRAVRLKAARLPVAPLRKCKEVKVEKPTADAEAYVFTPNMICAGGEKGMDSCKGDSGGAFAVQDPNDKTKFYAAGLVSWGPQCGTYGLYTRVKNYVDWIMKTMQENSTPRED
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Predicted Molecular Mass
76 kDa
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Molecular Weight
Approximately 55-60 kDa (light chain) & 32-35 kDa (heavy chain) & 76-96 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (239 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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