1. Recombinant Proteins
  2. CD Antigens Receptor Proteins
  3. Stem Cell CD Proteins Platelet CD Proteins Erythrocyte CD Proteins Epithelial cell CD Proteins Cell Adhesion Molecules (CAMs)
  4. CD324/E-Cadherin Cadherins
  5. E-Cadherin/Cadherin-1
  6. Cadherin-1/CD324 Protein, Rat (HEK293, His)

Cadherin-1/CD324 Protein, Rat (HEK293, His)

Cat. No.: HY-P74367
SDS COA Handling Instructions

Cadherin-1/CD324 protein, a calcium-dependent adhesion molecule, mediates cell processes. It interacts homophilically with other cadherin molecules, sorting and organizing cell types. CDH1 regulates adhesion, mobility, and proliferation of epithelial cells. It serves as an integrin ligand, suppressing invasion and promoting non-amyloidogenic degradation. CDH1 inhibits APP C99 and C83 production. Cadherin-1/CD324 Protein, Rat (HEK293, His) is the recombinant rat-derived Cadherin-1/CD324 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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  • Documentation

Description

Cadherin-1/CD324 protein, a calcium-dependent adhesion molecule, mediates cell processes. It interacts homophilically with other cadherin molecules, sorting and organizing cell types. CDH1 regulates adhesion, mobility, and proliferation of epithelial cells. It serves as an integrin ligand, suppressing invasion and promoting non-amyloidogenic degradation. CDH1 inhibits APP C99 and C83 production. Cadherin-1/CD324 Protein, Rat (HEK293, His) is the recombinant rat-derived Cadherin-1/CD324 protein, expressed by HEK293 , with C-His labeled tag.

Background

Cadherin-1/CD324 protein, a calcium-dependent cell adhesion molecule, plays a pivotal role in various cellular processes. By preferentially interacting with other cadherin molecules of the same type in a homophilic manner, CDH1 contributes to the sorting and organization of different cell types within tissues. Furthermore, it is involved in regulating cell-cell adhesions, mobility, and proliferation of epithelial cells. CDH1 also serves as a ligand for integrin alpha-E/beta-7 and exhibits a potent suppressor role in invasion. Additionally, it promotes the non-amyloidogenic degradation of Abeta precursors and exerts a strong inhibitory effect on the production of APP C99 and C83.

Biological Activity

Measured by the ability of the immobilized protein to support the adhesion of MCF-7 cells. When 5×104cells/well are added to Rat Cadherin-1/CD324 Protein, cell adhesion is enhanced in a dose dependent manner after 30 minutes at 37℃. The ED50 for this effect is 0.3603 μg/mL, corresponding to a specific activity is 2.775×103 U/mg.

  • Measured by the ability of the immobilized protein to support the adhesion of MCF-7 cells. When 5×104 cells/well are added to Rat Cadherin-1/CD324 Protein, cell adhesion is enhanced in a dose dependent manner after 30 minutes at 37℃. The ED50 for this effect is 0.3603 μg/mL, corresponding to a specific activity is 2.775×103 U/mg.
Species

Rat

Source

HEK293

Tag

C-10*His

Accession

Q9R0T4 (Q24-A713)

Gene ID
Molecular Construction
N-term
CADH1 (Q24-A713)
Accession # Q9R0T4
His
C-term
Synonyms
Cadherin-1; CDH1; CAM 120/80; E-cadherin; CD324
AA Sequence

QPESESDSCRPGFSSEVYTFLVPERHLERGHILGRVKFEGCTGRPRTAFFSEDSRFKVSTDGVITVKRHLKLHKLETSFLVHAWDSSYRKLSTKVTLKSLGHHHHRHHHRDPVSESNPELLTFPSFHQGLRRQKRDWVIPPINCPENQKGEFPQRLVQIKSNRDKETTVFYSITGPGADKPPVGVFIIERETGWLKVTQPLDREAIDKYLLYSHAVSSNGEAVEDPMEIVVTVTDQNDNRPEFIQEVFEGSVAEGALPGTSVMQVSATDADDDINTYNAAIAYTILSQDPELPHKNMFTVNRDTGVISVVTSGLDRESYPTYTLVVQAADLQGEGLSTTAKAVITVKDINDNAPIFNPSTYQGQVLENEVGARIATLKVTDDDAPNTPAWNAVYTVVNDPDHQFTVITDPKTNEGILKTAKGLDFEAKQQYILHVTVENEEPFEGSLVPSTATVTVDVVDVNEAPIFVPAEKRVEVPEDFGVGLEIASYTAREPDTFMEQKITYRIWRDTANWLEINPETGVISTRAEMDREDSEHVKNSTYTALIIATDDGSPIATGTGTLLLVLSDVNDNAPIPEPRNMQFCQRNPKPHVITILDPDLPPNTSPFTAELTHGASVNWTIEYNDAEQESLILQPRKDLEIGEYKINLKLSDNQNKDQVTTLEVHVCDCEGTVNNCMKAISLEAGLQVPA

Molecular Weight

Approximately 80 kDa due to the glycosylation.

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Cadherin-1/CD324 Protein, Rat (HEK293, His)
Cat. No.:
HY-P74367
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