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Cadherin-1/CD324 Protein, Rat (HEK293, His)

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Based on 1 Customer Validation

Cadherin-1/CD324 protein, a calcium-dependent adhesion molecule, mediates cell processes. It interacts homophilically with other cadherin molecules, sorting and organizing cell types. CDH1 regulates adhesion, mobility, and proliferation of epithelial cells. It serves as an integrin ligand, suppressing invasion and promoting non-amyloidogenic degradation. CDH1 inhibits APP C99 and C83 production. Cadherin-1/CD324 Protein, Rat (HEK293, His) is the recombinant rat-derived Cadherin-1/CD324 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Rat
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Cadherin-1/CD324 Protein, Rat (HEK293, His)
  • Experimental Validation Results for Cadherin-1/CD324 Protein, Rat (HEK293, His)
  • Cadherin-1/CD324 Protein, Rat (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

Cadherin-1/CD324 protein, a calcium-dependent adhesion molecule, mediates cell processes. It interacts homophilically with other cadherin molecules, sorting and organizing cell types. CDH1 regulates adhesion, mobility, and proliferation of epithelial cells. It serves as an integrin ligand, suppressing invasion and promoting non-amyloidogenic degradation. CDH1 inhibits APP C99 and C83 production. Cadherin-1/CD324 Protein, Rat (HEK293, His) is the recombinant rat-derived Cadherin-1/CD324 protein, expressed by HEK293 , with C-His labeled tag.

Background

Cadherin-1/CD324 protein, a calcium-dependent cell adhesion molecule, plays a pivotal role in various cellular processes. By preferentially interacting with other cadherin molecules of the same type in a homophilic manner, CDH1 contributes to the sorting and organization of different cell types within tissues. Furthermore, it is involved in regulating cell-cell adhesions, mobility, and proliferation of epithelial cells. CDH1 also serves as a ligand for integrin alpha-E/beta-7 and exhibits a potent suppressor role in invasion. Additionally, it promotes the non-amyloidogenic degradation of Abeta precursors and exerts a strong inhibitory effect on the production of APP C99 and C83.

Verified Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of MCF-7 cells. When 5×104cells/well are added to Rat Cadherin-1/CD324 Protein, cell adhesion is enhanced in a dose dependent manner after 30 minutes at 37℃. The ED50 for this effect is 0.3603 μg/mL, corresponding to a specific activity is 2.775×103 U/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by the ability of the immobilized protein to support the adhesion of MCF-7 cells. When 5×104 cells/well are added to Rat Cadherin-1/CD324 Protein, cell adhesion is enhanced in a dose dependent manner after 30 minutes at 37℃. The ED50 for this effect is 0.3603 μg/mL, corresponding to a specific activity is 2.775×103 U/mg.

Technical Parameters

  • Species Rat
  • Source HEK293
  • Tag C-10*His
  • Accession

    Q9R0T4 (Q24-A713)

  • Gene ID
    83502  [NCBI]
  • Molecular Construction
    • N-term
    • CADH1 (Q24-A713)
      Accession # Q9R0T4
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    CDH1; CDHE; Prev. UVO; Epididymis Secretory Sperm Binding Protein; Uvomorulin; Cadherin 1, E-Cadherin (Epithelial); Epithelial Cadherin; Cell-CAM 120/80; E-Cadherin; CD324 Antigen; Cadherin-1; Arc-1; CD324; BCDS1; Cadherin 1, Type 1, E-Cadherin (Epithelia

  • AA Sequence

    QPESESDSCRPGFSSEVYTFLVPERHLERGHILGRVKFEGCTGRPRTAFFSEDSRFKVSTDGVITVKRHLKLHKLETSFLVHAWDSSYRKLSTKVTLKSLGHHHHRHHHRDPVSESNPELLTFPSFHQGLRRQKRDWVIPPINCPENQKGEFPQRLVQIKSNRDKETTVFYSITGPGADKPPVGVFIIERETGWLKVTQPLDREAIDKYLLYSHAVSSNGEAVEDPMEIVVTVTDQNDNRPEFIQEVFEGSVAEGALPGTSVMQVSATDADDDINTYNAAIAYTILSQDPELPHKNMFTVNRDTGVISVVTSGLDRESYPTYTLVVQAADLQGEGLSTTAKAVITVKDINDNAPIFNPSTYQGQVLENEVGARIATLKVTDDDAPNTPAWNAVYTVVNDPDHQFTVITDPKTNEGILKTAKGLDFEAKQQYILHVTVENEEPFEGSLVPSTATVTVDVVDVNEAPIFVPAEKRVEVPEDFGVGLEIASYTAREPDTFMEQKITYRIWRDTANWLEINPETGVISTRAEMDREDSEHVKNSTYTALIIATDDGSPIATGTGTLLLVLSDVNDNAPIPEPRNMQFCQRNPKPHVITILDPDLPPNTSPFTAELTHGASVNWTIEYNDAEQESLILQPRKDLEIGEYKINLKLSDNQNKDQVTTLEVHVCDCEGTVNNCMKAISLEAGLQVPA

  • Molecular Weight

    Approximately 79 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by the ability of the immobilized protein to support the adhesion of MCF-7 cells. When 5×104 cells/well are added to Rat Cadherin-1/CD324 Protein, cell adhesion is enhanced in a dose dependent manner after 30 minutes at 37℃. The ED50 for this effect is 0.3603 μg/mL, corresponding to a specific activity is 2.775×103 U/mg.

  • Cadherin-1/CD324 Protein, Rat (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Cadherin-1/CD324 Protein, Rat (HEK293, His)
  • Experimental Validation Results for Cadherin-1/CD324 Protein, Rat (HEK293, His)
  • Cadherin-1/CD324 Protein, Rat (HEK293, His) Product Image