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Cadherin-13 Protein, Mouse (HEK293, His)

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Based on 1 Customer Validation

Cadherin-13 proteins are calcium-dependent cell adhesion molecules that exhibit a preference for homogeneous interactions, promote connections between adjacent cells, and may play a role in cell type sorting. Cadherin-13 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Cadherin-13 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Cadherin-13 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Cadherin-13 Protein, Mouse (HEK293, His)
  • Cadherin-13 Protein, Mouse (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Cadherin-13 proteins are calcium-dependent cell adhesion molecules that exhibit a preference for homogeneous interactions, promote connections between adjacent cells, and may play a role in cell type sorting. Cadherin-13 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Cadherin-13 protein, expressed by HEK293 , with C-His labeled tag.

Background

Cadherin-13, a calcium-dependent cell adhesion protein, stands out for its unique features in comparison to classical cadherins. Unlike the typical cadherin EC1 domain strand-swapping mechanism for homodimerization, Cadherin-13 achieves this through a distinct homophilic adhesive interface, connecting two elongated EC1-EC2 domains near their Ca2+-binding sites. This arrangement forms a tetrahedral, X-like shape, showcasing a different mode of homodimerization. This protein is implicated in potentially acting as a negative regulator of neural cell growth, and its ability to preferentially interact with itself in a homophilic manner suggests a role in contributing to the sorting of heterogeneous cell types within neural tissues.

Verified Bioactivity

Measured by the ability of the immobilized protein to inhibit the adhesion of HUVEC human umbilical vein endothelial cells. When 5 x 104 cells/well are added to Recombinant Mouse Cadherin-13 coated plates, cell adhesion is inhibited in a dose dependent manner after 2.5 hours at 37 °C. The ED50 for this effect is 4.025 μg/mL, corresponding to a specific activity is 248.447 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by the ability of the immobilized protein to inhibit the adhesion of HUVEC human umbilical vein endothelial cells.. When 5 x 104 cells/well are added to Recombinant Mouse Cadherin-13 coated plates, cell adhesion is inhibited in a dose dependent manner after 2.5 hours at 37 °C. The ED50 for this effect is 4.025 μg/mL, corresponding to a specific activity is 248.447 units/mg.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-10*His
  • Accession

    Q9WTR5 (D23-A692)

  • Gene ID
    12554  [NCBI]
  • Molecular Construction
    • N-term
    • Cadherin-13 (D23-A692)
      Accession # Q9WTR5
    • His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    CDH13; Cadherin-13; Cadherin 13; T-Cad; CDHH; P105; T-Cadherin; Cadherin 13, H-Cadherin (Heart); H-Cadherin (Heart); Truncated Cadherin; Heart Cadherin; H-Cadherin

  • AA Sequence

    DDLECTPGFQRKVLHIHQPAEFIEDQPVLNLTFNDCKGNEKLHYEVSSPHFKVNSDGTLVALRNITAVGRTLFVHARTPHAEDMAELVIVGGKDIQGSLQDIFKFARTSPVPRQKRSIVVSPILIPENQRQPFPRDVGKVVDSDRPEGSKFRLTGKGVDQDPKGTFRINENTGSVSVTRTLDRETIATYQLYVETTDASGKTLEGPVPLEVIVIDQNDNRPIFREGPYIGHVMEGSPTGTTVMRMTAFDADDPATDNALLRYNIRQQTPDKPSPNMFYIDPEKGDIVTVVSPALLDRETLENPKYELIIEAQDMAGLDVGLTGTATATIVIDDKNDHSPKFTKKEFQATVEEGAVGVIVNLTVEDKDDPTTGAWRAAYTIINGNPGQSFEIHTNPQTNEGMLSVVKPLDYEISAFHTLLIKVENEDPLVPDVSYGPSSTATVHITVLDVNEGPVFYPDPMMVTKQENISVGSVLLTVNATDPDSLQHQTIRYSIYKDPAGWLSINPINGTVDTTAVLDRESPFVHNSVYTALFLAIDSGNPPATGTGTLLITLEDINDNAPVIYPTVAEVCDDARNLSVVILGASDKDLHPNTDPFKFEIHKQTVPDKVWKISKINNTHALVSLLQNLNKANYNLPIMVTDSGKPPMTNITDLRVQVCSCKNSKVDCNGA

  • Molecular Weight

    Approximately 90-110 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by the ability of the immobilized protein to inhibit the adhesion of HUVEC human umbilical vein endothelial cells.. When 5 x 104 cells/well are added to Recombinant Mouse Cadherin-13 coated plates, cell adhesion is inhibited in a dose dependent manner after 2.5 hours at 37 °C. The ED50 for this effect is 4.025 μg/mL, corresponding to a specific activity is 248.447 units/mg.

  • Cadherin-13 Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Cadherin-13 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Cadherin-13 Protein, Mouse (HEK293, His)
  • Cadherin-13 Protein, Mouse (HEK293, His) Product Image