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Cadherin-12/CDH12 Protein, Human (HEK293, His)

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Based on 1 publication(s) in Google Scholar

Cadherin-12/CDH12 is a calcium-dependent cell adhesion protein in the cadherin family known for its preferential homophilic interactions (binding to itself between connecting cells). This interaction is shown to play a crucial role in mediating cell adhesion and may play a key role in sorting heterogeneous cell types. Cadherin-12/CDH12 Protein, Human (HEK293, His) is the recombinant human-derived Cadherin-12/CDH12 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Cadherin-12/CDH12 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Cadherin-12/CDH12 is a calcium-dependent cell adhesion protein in the cadherin family known for its preferential homophilic interactions (binding to itself between connecting cells). This interaction is shown to play a crucial role in mediating cell adhesion and may play a key role in sorting heterogeneous cell types. Cadherin-12/CDH12 Protein, Human (HEK293, His) is the recombinant human-derived Cadherin-12/CDH12 protein, expressed by HEK293 , with C-His labeled tag.

Background

Cadherin-12/CDH12 protein, belonging to the cadherin family, operates as a calcium-dependent cell adhesion protein. Known for its preferential homophilic interaction, Cadherin-12/CDH12 engages in binding with itself between connecting cells. This homophilic cadherin interaction implies a significant role in mediating cell adhesion and may play a crucial part in the intricate process of sorting heterogeneous cell types. Through its calcium-dependent adhesive properties, Cadherin-12/CDH12 likely contributes to the establishment of selective connections between cells, thereby participating in the dynamic regulation of cellular interactions and tissue organization.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    P55289-1/NP_001304156.1 (Q24-A605)

  • Gene ID
    1010
  • Molecular Construction
    • N-term
    • CDH12 (Q24-A605)
      Accession # P55289
    • His
    • C-term
  • Protein Length

    Extracellular Domain (with propeptide)

  • Synonyms

    CDH12; Cadherin 12, Type 2 (N-Cadherin 2); Cadherin 12; Cadherin, Neural Type, 2; Br-Cadherin; Neuronal Cadherin 2; CDHB; Brain-Cadherin; Neural Type Cadherin 2; Brain Cadherin; N-Cadherin 2; BR-Cadherin; Cadherin-12

  • AA Sequence

    QPQPQQTLATEPRENVIHLPGQRSHFQRVKRGWVWNQFFVLEEYVGSEPQYVGKLHSDLDKGEGTVKYTLSGDGAGTVFTIDETTGDIHAIRSLDREEKPFYTLRAQAVDIETRKPLEPESEFIIKVQDINDNEPKFLDGPYVATVPEMSPVGAYVLQVKATDADDPTYGNSARVVYSILQGQPYFSIDPKTGVIRTALPNMDREVKEQYQVLIQAKDMGGQLGGLAGTTIVNITLTDVNDNPPRFPKSIFHLKVPESSPIGSAIGRIRAVDPDFGQNAEIEYNIVPGDGGNLFDIVTDEDTQEGVIKLKKPLDFETKKAYTFKVEASNLHLDHRFHSAGPFKDTATVKISVLDVDEPPVFSKPLYTMEVYEDTPVGTIIGAVTAQDLDVGSSAVRYFIDWKSDGDSYFTIDGNEGTIATNELLDRESTAQYNFSIIASKVSNPLLTSKVNILINVLDVNEFPPEISVPYETAVCENAKPGQIIQIVSAADRDLSPAGQQFSFRLSPEAAIKPNFTVRDFRNNTAGIETRRNGYSRRQQELYFLPVVIEDSSYPVQSSTNTMTIRVCRCDSDGTILSCNVEA

  • Predicted Molecular Mass

    66 kDa

  • Molecular Weight

    Approximately 80-85 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 80%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of 50 mM sodium citrate, 50 mM NaCl, 2 mM CaCl2, pH 6.0, 5% trehalose, 5% mannitol and 0.01% Tween 80.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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