CASPR2 Protein, Mouse (HEK293, His)
Based on 1 Customer Validation
CASPR2 protein has PDZ domain binding activity and plays a crucial role in synaptic transmission, nervous system development and synaptic organization. It is essential for processes such as glutamatergic neuronal differentiation and limbic system development, and is located in cellular components such as cerebellar synapses and presynaptic active zone membranes. CASPR2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CASPR2 protein, expressed by HEK293 , with C-10*His labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
CASPR2 protein has PDZ domain binding activity and plays a crucial role in synaptic transmission, nervous system development and synaptic organization. It is essential for processes such as glutamatergic neuronal differentiation and limbic system development, and is located in cellular components such as cerebellar synapses and presynaptic active zone membranes. CASPR2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CASPR2 protein, expressed by HEK293 , with C-10*His labeled tag.
CASPR2 Protein exhibits PDZ domain binding activity and is involved in diverse processes such as chemical synaptic transmission, nervous system development, and synapse organization. It plays a crucial role upstream of or within processes like glutamatergic neuron differentiation, limbic system development, and neuron projection development. CASPR2 is located in various cellular components, including the cerebellar granule cell to Purkinje cell synapse, main axon, and presynaptic active zone membrane, and serves as an integral component of the membrane and the voltage-gated potassium channel complex. Active in dendrites and excitatory synapses, CASPR2 is prominently expressed in the central nervous system and neural retina. It has been studied in the context of autism spectrum disorder and cortical dysplasia-focal epilepsy syndrome. Additionally, its human ortholog(s), particularly CNTNAP2 (contactin-associated protein 2), are implicated in diseases such as Pitt-Hopkins syndrome, autism spectrum disorder, communication disorder, cortical dysplasia-focal epilepsy syndrome, and social phobia. The gene demonstrates biased expression in CNS E18 (RPKM 4.2), whole brain E14.5 (RPKM 3.7), and seven other tissues, highlighting its involvement in critical developmental and neurological processes across various tissues.
Immobilized Recombinant Mouse CASPR2 at 0.5 μg/mL (100 μL/well) can bind Biotinylated Recombinant Human Contactin-1 Protein. The ED50 for this effect is 0.7407 μg/mL.
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-10*His
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Accession
NP_001004357.2 (A28-S1262)
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Molecular Construction
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N-term
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CASPR2 (A28-S1262)
Accession # NP_001004357.2 -
10*His
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C-term
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Protein Length
Partial
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Synonyms
CNTNAP2; Contactin Associated Protein-Like 2 Isoform E; Contactin Associated Protein 2; Contactin Associated Protein-Like 2 Isoform B; KIAA0868; Contactin Associated Protein-Like 2 Isoform G; Caspr2; Contactin Associated Protein-Like 2 Isoform C; NRXN4; C
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AA Sequence
APSTFQKCDEPLISGLPHVSFSSSSSLSSSYAPGYAKINKRGGAGGWSPSDSDHYQWLQVDFGNRKQISAIATQGRYSSSDWVTQYRMLYSDTGRNWKPYHQDGNIWAFPGNVNSDSVVRHDLQHAVVARYVRVVPLDWNGEGHIGLRAEVYGCAYWADVINFDGHGVLPYRFRNKKMKTLKDVIALKFKTSESEGVLLHGEGQQGDYITLELKKAKLVLSLNLGSNQLGPIYGHTSVTSGSLLDDHHWHSVLIERQGRSINLTLDRSMQHFRTNGEFDYLDLDYEITFGGIPFSGKPSSSNRKNFKGCMESINYNGVNITDLARRKKLEPSNMGNLSFSCVEPYTVPVFFNATSYLEVPGRLNQDLFSVSFQFRTWNPSGLLLFSHFADNLGNVEIDLVESKVGVHINNTQTKTSQIDISSGSGLNDGQWHEVRFLAKENFAVLTIDGDEASAVRTNSPLQVKTGEKYFFGGFLNHMNNASYSALQPSFQGCMQLIQVDDQLVNLYEVAHRKPGSFANVTIDMCAIIDRCVPNHCEHGGKCSQTWDSFKCTCDETGYSGATCHNSIYEPSCEAYKHLGQTSNYYWIDPDGSGPLGPLKVYCNMTEDKVWTIVSHDLQMQTTVVGYNPEKYSVTQLIYSASMDQISAITSSAEYCEQYVSYFCRMSRLLNTPDGSPYTWWVGKANEKHYYWGGSEPGIQKCACGIERNCTDPKYYCNCDADYKQWRKDAGFLSYKDHLPVSQVVVGDTDRQGSEAKLSVGPLRCQGDRNYWNAASFPNPSSYLHFSTFQGETSADISFYFKTLIPRGVFLENLGNTDFIKLELKSATEVSFSFDVGNGPVEIVVRSPSPLNDDQWHRVTAERNVKQASLQVDRLPQQIRKAPTEGHTRLELYSQLFVGGAGGQQGFLGCIRSLRMNGVTLDLEERAKVTSGFKSGCSGHCTSYGANCENGGKCIEKYHGYSCDCSNTAYDGTFCNKDVGAFFEEGMWLRYNFQAPAVTARDTGSRAENSADQQQHLAPDLAQEQIHFSFSTTKAPCILLYVSSLTTDFLAVLVKPTGNLQIRYNLGGTREPFNIDVDHRNMANGQPHSVNITRHERTIILKLDHYPAVGYHLPSSSDTLFNSPKSLFLGKVIETGKIDQEIHKYNTPGFTGCLSRVQFNHIAPLKAALRQTNASAHVHIQGELVESNCGASPLTLSPMSSATDPWHLDHLDSASADFPYNPGQGQAIRNGVNRNS
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Molecular Weight
Approximately 140-150 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (243 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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