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CCN2/CTGF Protein, Mouse (GST)

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Based on 2 publication(s) in Google Scholar

CCN2/CTGF protein is a major connective tissue mitochondrial attractant secreted by vascular endothelial cells and promotes chondrocyte proliferation and differentiation. It mediates heparin- and divalent cation-dependent cell adhesion in various cell types, including fibroblasts, myofibroblasts, endothelial cells, and epithelial cells. CCN2/CTGF Protein, Mouse (GST) is the recombinant mouse-derived CCN2/CTGF protein, expressed by E. coli , with N-GST labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for CCN2/CTGF Protein, Mouse (GST)
  • CCN2/CTGF Protein, Mouse (GST) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

CCN2/CTGF protein is a major connective tissue mitochondrial attractant secreted by vascular endothelial cells and promotes chondrocyte proliferation and differentiation. It mediates heparin- and divalent cation-dependent cell adhesion in various cell types, including fibroblasts, myofibroblasts, endothelial cells, and epithelial cells. CCN2/CTGF Protein, Mouse (GST) is the recombinant mouse-derived CCN2/CTGF protein, expressed by E. coli , with N-GST labeled tag.

Background

CCN2/CTGF protein, a major connective tissue mitoattractant, is secreted by vascular endothelial cells. It plays a crucial role in promoting the proliferation and differentiation of chondrocytes. Additionally, CCN2/CTGF mediates heparin- and divalent cation-dependent cell adhesion in various cell types, including fibroblasts, myofibroblasts, endothelial cells, and epithelial cells. Furthermore, it enhances fibroblast growth factor-induced DNA synthesis. CCN2/CTGF exists as a monomer and interacts with TSKU.

Verified Bioactivity

Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. The ED50 for this effect is 1.824 μg/mL.

Technical Parameters

  • Species Mouse
  • Source E. coli
  • Tag N-GST
  • Accession

    P29268 (Q26-Y344)

  • Gene ID
    14219  [NCBI]
  • Molecular Construction
    • N-term
    • GST
    • Ccn2 (Q26-Y344)
      Accession # P29268
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    CCN2; CCN Family Member 2; Prev. CTGF; IBP-8; IGFBP8; SEMDLSL; Connective Tissue Growth Factor; IGFBP-8; Hypertrophic Chondrocyte-Specific Protein 24; NOV2; Insulin-Like Growth Factor-Binding Protein 8; KMD; IGF-Binding Protein 8; Cellular Communication N

  • AA Sequence

    QDCSAQCQCAAEAAPHCPAGVSLVLDGCGCCRVCAKQLGELCTERDPCDPHKGLFCDFGSPANRKIGVCTAKDGAPCVFGGSVYRSGESFQSSCKYQCTCLDGAVGCVPLCSMDVRLPSPDCPFPRRVKLPGKCCEEWVCDEPKDRTAVGPALAAYRLEDTFGPDPTMMRANCLVQTTEWSACSKTCGMGISTRVTNDNTFCRLEKQSRLCMVRPCEADLEENIKKGKKCIRTPKIAKPVKFELSGCTSVKTYRAKFCGVCTDGRCCTPHRTTTLPVEFKCPDGEIMKKNMMFIKTCACHYNCPGDNDIFESLYYRKMY

  • Predicted Molecular Mass

    62 kDa

  • Molecular Weight

    Approximately 65 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
2.Lyophilized from a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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    MedChemExpress Validation
     Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. The ED50 for this effect is 1.824 μg/mL, corresponding to a specific activity is 5.48×102 units/mg.

  • CCN2/CTGF Protein, Mouse (GST) Product Image
    1/16
  • Experimental Validation Results for CCN2/CTGF Protein, Mouse (GST)
  • CCN2/CTGF Protein, Mouse (GST) Product Image