CD162/PSGL-1 Protein, Mouse (HEK293, His)
The CD162/PSGL-1 protein is an SLe(x)-type proteoglycan that mediates rapid rolling of leukocytes through high-affinity interactions with E- and P-selectin, thereby promoting early inflammatory stages.CD162 forms homodimers linked by disulfide bonds and interacts with selectins through the lectin/EGF domain.CD162/PSGL-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD162/PSGL-1 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The CD162/PSGL-1 protein is an SLe(x)-type proteoglycan that mediates rapid rolling of leukocytes through high-affinity interactions with E- and P-selectin, thereby promoting early inflammatory stages.CD162 forms homodimers linked by disulfide bonds and interacts with selectins through the lectin/EGF domain.CD162/PSGL-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD162/PSGL-1 protein, expressed by HEK293 , with C-His labeled tag.
Background
CD162, also known as P-selectin glycoprotein ligand-1 (PSGL-1), functions as an SLe(x)-type proteoglycan and plays a crucial role in the early stages of inflammation by mediating rapid leukocyte rolling over vascular surfaces through high-affinity, calcium-dependent interactions with E- and P-selectins. This interaction is pivotal for the initial capture of leukocytes. CD162 forms homodimers linked by disulfide bonds and interacts with P- and E-selectins through their lectin/EGF domains. For high-affinity binding to P-selectin, sialyl Lewis X glycan modification and tyrosine sulfation, likely on Tyr-54, are required. Dimerization appears dispensable for P-selectin binding. Additionally, CD162 interacts with sorting nexin 20 (SNX20) and mediates SYK activation downstream of P-selectin/SELP binding. Furthermore, CD162 interacts with moesin (MSN) and spleen tyrosine kinase (SYK), contributing to downstream signaling events. It also engages with HAVCR1, revealing its involvement in diverse cellular interactions.
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-8*His
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Accession
Q62170 (Q42-C307)
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Molecular Construction
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N-term
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PSGL-1 (Q42-C307)
Accession # Q62170 -
8*His
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C-term
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Protein Length
Partial Extracellular Domain
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Synonyms
SELPLG; Cutaneous Lymphocyte-Associated Associated Antigen; Selectin P Ligand; CD162 Antigen; PSGL-1; PSGL1; CD162; CLA; P-Selectin Glycoprotein Ligand 1
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AA Sequence
QVVGDDDFEDPDYTYNTDPPELLKNVTNTVAAHPELPTTVVMLERDSTSAGTSERATEKIATTDPTAPGTGGTAVGMLSTDSATQWSLTSVETVQPASTEVETSQPAPMEAETSQPAPMEAETSQPAPMEAETSQPAPMEADTSQPAPMEAETSQPAPNEAETSKPAPTEAETSKPAPTEAETTQLPRIQAVKTLFTTSAATEVPSTEPTTMETASTESNESTIFLGPSVTHLPDSGLKKGLIVTPGNSPAPTLPGSSDLIPVKQC
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Predicted Molecular Mass
28.7 kDa
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Molecular Weight
Approximately 68-108 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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