FITC-Labeled CD19 Protein, Human (HEK293, His)
Based on 1 Customer Validation
The CD19 protein is a B cell antigen receptor coreceptor that enhances downstream signaling pathways, lowers the activation threshold, and helps B cells respond to antigens. It activates phosphatidylinositol 3-kinase and mobilizes intracellular Ca(2+). CD19 Protein, Human (FITC, HEK293, His) is the recombinant human-derived CD19 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 1 year from date of receipt, protect from light. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The CD19 protein is a B cell antigen receptor coreceptor that enhances downstream signaling pathways, lowers the activation threshold, and helps B cells respond to antigens. It activates phosphatidylinositol 3-kinase and mobilizes intracellular Ca(2+). CD19 Protein, Human (FITC, HEK293, His) is the recombinant human-derived CD19 protein, expressed by HEK293 , with C-His labeled tag.
Background
The CD19 Protein serves as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes, playing a pivotal role in decreasing the threshold for activation of downstream signaling pathways and facilitating B-cell responses to antigens. It activates signaling pathways leading to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores. Although not required for early steps during B cell differentiation in the blood marrow, CD19 is essential for the normal differentiation of B-1 cells. Moreover, it is crucial for normal B cell differentiation and proliferation in response to antigen challenges, influencing serum immunoglobulin levels and the production of high-affinity antibodies in response to antigen challenge. CD19 forms complexes with CR2/CD21, CD81, and IFITM1/CD225 in the membrane of mature B-cells. It interacts directly with CD81, a crucial interaction for trafficking and compartmentalization of the CD19 receptor on the cell surface of activated B cells. Additionally, CD19 interacts with VAV, GRB2, SOS, PLCG2, LYN, and the regulatory p85 subunit of phosphatidylinositol 3-kinase when phosphorylated on specific tyrosine residues.
Verified Bioactivity
1.Immobilized FITC-Compatible Human CD19, His Tag at 2μg/mL (100μl/well) on the plate. Dose response curve for Anti-CD19 Antibody, hFc Tag with the EC50 of 9.3ng/mL determined by ELISA.
2.2e5 of 293 CAR cells transfected with anti-CD19-scFv were stained with 100 μL of 10 μg/mL of FITC Labeled Human CD19, His Tag and negative control protein respectively, and non-transfected 293 cells were used as a control, FITC signal was used to evaluate the binding activity.
3.5e5 of PBMCs were stained with FITC-Labeled Human CD19 (20-291), His Tag and anti-CD3 antibody, washed and then analyzed with FACS. PE signal was used to evaluate the expression of CD3+ T cells in PBMCs, and FITC signal was used to evaluate the non-specific binding activity to PBMCs.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-8*His
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Accession
P15391-1 (P20-K291)
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Gene ID930
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Molecular Construction
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N-term
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CD19 (P20-K291)
Accession # P15391-1 -
8*His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
CD19; CDNA FLJ60916, Highly Similar To B-Lymphocyte Antigen CD19; CD19 Molecule; CDNA FLJ54955, Highly Similar To B-Lymphocyte Antigen CD19; B-Lymphocyte Surface Antigen B4; CD19 Antigen, Isoform CRA_f; T-Cell Surface Antigen Leu-12; CD19 Antigen, Isoform
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AA Sequence
PEEPLVVKVEEGDNAVLQCLKGTSDGPTQQLTWSRESPLKPFLKLSLGLPGLGIHMRPLAIWLFIFNVSQQMGGFYLCQPGPPSEKAWQPGWTVNVEGSGELFRWNVSDLGGLGCGLKNRSSEGPSSPSGKLMSPKLYVWAKDRPEIWEGEPPCLPPRDSLNQSLSQDLTMAPGSTLWLSCGVPPDSVSRGPLSWTHVHPKGPKSLLSLELKDDRPARDMWVMETGLLLPRATAQDAGKYYCHRGNLTMSFHLEITARPVLWHWLLRTGGWK
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Molecular Weight
Approximately 68-72 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 10% trehalose.
Please refer to the lot-specific COA for specific buffer information.
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It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 1 year from date of receipt, protect from light. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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