Copy Link Print Information

CD1B-B2M Heterodimer Protein, Rhesus Macaque (HEK293, His)

Customer Review

Based on 1 Customer Validation

CD1B belongs to family 1 of the transmembrane glycoprotein CD1 family and plays a critical role in immune surveillance and response. As a key antigen-presenting molecule, CD1B protein has shown the ability to bind both self and non-self-lipid and glycolipid antigens. CD1B is a potential prognostic biomarker associated with tumor mutation burden and promotes antitumor immunity in lung adenocarcinoma. CD1B-B2M Heterodimer Protein, Rhesus Macaque (HEK293, His) is a recombinant protein dimer complex containing Rhesus Macaque-derived CD1B-B2M Heterodimer protein, expressed by HEK293 , with C-His labeled tag. CD1B-B2M Heterodimer Protein, Rhesus Macaque (HEK293, His), has molecular weight of ~38-45 & 12 KDa.

For research use only. We do not sell to patients.
  • Species: Rhesus Macaque
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for CD1B-B2M Heterodimer Protein, Rhesus Macaque (HEK293, His)
  • Experimental Validation Results for CD1B-B2M Heterodimer Protein, Rhesus Macaque (HEK293, His)
  • CD1B-B2M Heterodimer Protein, Rhesus Macaque (HEK293, His) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

CD1B belongs to family 1 of the transmembrane glycoprotein CD1 family and plays a critical role in immune surveillance and response. As a key antigen-presenting molecule, CD1B protein has shown the ability to bind both self and non-self-lipid and glycolipid antigens. CD1B is a potential prognostic biomarker associated with tumor mutation burden and promotes antitumor immunity in lung adenocarcinoma. CD1B-B2M Heterodimer Protein, Rhesus Macaque (HEK293, His) is a recombinant protein dimer complex containing Rhesus Macaque-derived CD1B-B2M Heterodimer protein, expressed by HEK293 , with C-His labeled tag. CD1B-B2M Heterodimer Protein, Rhesus Macaque (HEK293, His), has molecular weight of ~38-45 & 12 KDa.

Background

CD1B belongs to Group 1 of the transmembrane glycoprotein CD1 family. The CD1 molecule is expressed on the surface of many different human antigen-presenting cells (DC, monocytes, and some thymus cells). The CD1B protein, as a key antigen-presenting molecule, has shown the ability to bind both self and non-self lipid and glycolipid antigens, thereby facilitating their presentation to T cell receptors on natural killer T cells. This protein forms a heterodimer complex with B2M (beta-2 microglobulin), which plays a crucial role in immune surveillance and response. Its interaction with saponin C further enhances its functional library, helping T cells to recognize and activate the complex process of various antigens. CD1B is a potential prognostic biomarker associated with tumor mutation burden and promotes antitumor immunity in lung adenocarcinoma[1][2][3].

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Rhesus Macaque CD1B-B2M Heterodimer at 2 μg/mL (100 μL/well) can bind Anti-CD1B Antibody, The ED50 for this effect is 1.538 μg/mL .

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized Rhesus Macaque CD1B-B2M Heterodimer at 2 μg/mL (100 μL/well) can bind Anti-CD1B Antibody, The ED50 for this effect is 1.538 μg/mL .

Technical Parameters

  • Species Rhesus Macaque
  • Source HEK293
  • Tag C-6*His
  • Accession

    NP_001139006 (E19-S303)&NP_001040602 (I21-M119)

  • Gene ID
    718955  [NCBI]&712428  [NCBI]
  • Synonyms

    CD1B; CD1B Antigen, B Polypeptide; Prev. CD1; Cortical Thymocyte Antigen CD1B; T-Cell Surface Glycoprotein CD1b; R1; CD1b Molecule; CD1b Antigen; B2M; Beta 2-Microglobulin; Beta-2-Microglobulin; Beta-2-Microglobin; Beta Chain Of MHC Class I Molecules; AMY

  • Molecular Weight

    Approximately 38-45 kDa & 12 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Rhesus Macaque CD1B-B2M Heterodimer at 2 μg/mL (100 μL/well) can bind Anti-CD1B Antibody, The ED50 for this effect is 1.538 μg/mL .

  • CD1B-B2M Heterodimer Protein, Rhesus Macaque (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for CD1B-B2M Heterodimer Protein, Rhesus Macaque (HEK293, His)
  • Experimental Validation Results for CD1B-B2M Heterodimer Protein, Rhesus Macaque (HEK293, His)
  • CD1B-B2M Heterodimer Protein, Rhesus Macaque (HEK293, His) Product Image