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CD316/IGSF8 Protein, Mouse (HEK293, His)

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Based on 1 Customer Validation

The CD316/IGSF8 protein plays a key role in functions related to CD81 and CD9, affecting oocyte fertilization and may affect HCV function. It regulates keratinocyte proliferation, acts as a negative regulator of cell motility, and binds to CD82 to inhibit prostate cancer cell migration. CD316/IGSF8 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD316/IGSF8 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for CD316/IGSF8 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for CD316/IGSF8 Protein, Mouse (HEK293, His)
  • CD316/IGSF8 Protein, Mouse (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The CD316/IGSF8 protein plays a key role in functions related to CD81 and CD9, affecting oocyte fertilization and may affect HCV function. It regulates keratinocyte proliferation, acts as a negative regulator of cell motility, and binds to CD82 to inhibit prostate cancer cell migration. CD316/IGSF8 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD316/IGSF8 protein, expressed by HEK293 , with C-His labeled tag.

Background

The CD316/IGSF8 protein appears to play a pivotal role in a variety of functions attributed to CD81 and CD9, such as oocyte fertilization and potential involvement in hepatitis C virus function. Additionally, it is implicated in regulating the proliferation and differentiation of keratinocytes and may act as a negative regulator of cell motility, suppressing T-cell mobility in coordination with CD81, associating with CD82 to suppress prostate cancer cell migration, and regulating epidermoid cell reaggregation and motility on laminin-5 with CD9 and CD81 as crucial linkers. CD316/IGSF8 might also contribute to integrin-dependent morphology and motility functions, participating in the regulation of neurite outgrowth and the maintenance of the neural network in the adult brain. Interactions with CD82, CD9/tetraspanin-29, integrin alpha-3/beta-1, and integrin alpha-4/beta-1 are suggested, with additional participation in a complex composed of CD9, PTGFRN, and CD81. Furthermore, direct interaction with CD81/tetraspanin-28 is noted, emphasizing the intricate network of associations that define the diverse roles of CD316/IGSF8 in cellular processes.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Mouse CD316 at 1 µg/mL can bind Biotinylated Mouse CD81. The ED50

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized Mouse CD316 at 1 µg/mL can bind Biotinylated Mouse CD81. The ED50 for this effect is 1.152 μg/mL.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-10*His
  • Accession

    Q8R366 (R26-T577)

  • Gene ID
    140559  [NCBI]
  • Molecular Construction
    • N-term
    • IGSF8 (R26-T577)
      Accession # Q8R366
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    IGSF8; CD81 Partner 3; Immunoglobulin Superfamily Member 8; LIR-D1; CD81P3; EWI-2; EWI2; KCT-4; PGRL; Immunoglobulin Superfamily, Member 8; CD316; CD316 Antigen; Keratinocytes-Associated Transmembrane Protein 4; IgSF8; Glu-Trp-Ile EWI Motif-Containing Pro

  • AA Sequence

    RQVHVPRGPLYRVAGTAVSISCNVSDYEGPAQQDFEWFMYRPEAPATSLGIVSTKDSQFSYAVFGPRVASGDLQVQRLKGDSVVLKIARLQAQDSGFYECYTPSTDTQYLGNYSAKVELRVLPDELQVSAAPPGPRGRQAATSPSRLTVHEGQELALGCLAQTKTKKHTHLSVSFGRAIPEAPVGRATLQEVVGLRSDMAVEAGAPYAERLASGELRLSKEGTDRYRMVVGGAQAGDSGTYHCTAAEWIQDPDGSWVQVAEKRAVLAHVDVQTLSSQLAVTVGPGERRIGPGEPLELLCNVSGALPPPGRHAAYSVGWEMAPAGAPGPGRLVAQLDTEGIGSLGPGYEDRHIAMEKVASRTYRLRLEAARPADAGTYRCLAKAYVRGSGTRLREAASARSRPLPVHVREEGVVLEAVAWLAGGTVYRGETASLLCNISVRGGPPGLRLAASWWVERPEEGELSSGPAQLVGGVGQDGVAELGVRPGGGPVSVELVGPRSHRLRLHGLGPEDEGIYHCAPSAWVQHADYSWYQAGSARSGPVTVYPYTHAVDT

  • Molecular Weight

    Approximately 66 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Mouse CD316 at 1 µg/mL can bind Biotinylated Mouse CD81. The ED50 for this effect is 1.152 μg/mL.

  • CD316/IGSF8 Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for CD316/IGSF8 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for CD316/IGSF8 Protein, Mouse (HEK293, His)
  • CD316/IGSF8 Protein, Mouse (HEK293, His) Product Image