Copy Link Print Information

CD34 Protein, Rat (HEK293, Fc)

Customer Review

Based on 1 Customer Validation

CD34 Protein is a transmembrane phosphoglycoprotein that exists on the surface of human and various animal cells. CD34 Protein is used as a cellular marker for hematopoietic stem cell sorting and as an islet endocrine cell marker. CD34 Protein plays an important role in cell adhesion, inflammatory cell chemotaxis, cell proliferation and differentiation, and enhancement of inflammatory response. CD34 Protein, Rat (HEK293, Fc) is the recombinant rat-derived CD34 protein, expressed by HEK293 , with C-hFc labeled tag.

For research use only. We do not sell to patients.
  • Species: Rat
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for CD34 Protein, Rat (HEK293, Fc)
  • Experimental Validation Results for CD34 Protein, Rat (HEK293, Fc)
  • CD34 Protein, Rat (HEK293, Fc) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

CD34 Protein is a transmembrane phosphoglycoprotein that exists on the surface of human and various animal cells. CD34 Protein is used as a cellular marker for hematopoietic stem cell sorting and as an islet endocrine cell marker. CD34 Protein plays an important role in cell adhesion, inflammatory cell chemotaxis, cell proliferation and differentiation, and enhancement of inflammatory response. CD34 Protein, Rat (HEK293, Fc) is the recombinant rat-derived CD34 protein, expressed by HEK293 , with C-hFc labeled tag.

Background

CD34 is a transmembrane phosphoglycoprotein present on the surface of human and various animal cells, and it was first described in hematopoietic stem cells as an intercellular adhesion factor. CD34 is used as a cellular marker for hematopoietic stem cell sorting and as an islet endocrine cell marker. CD34 can mediate the attachment of hematopoietic stem cells to bone marrow extracellular matrix or directly to stromal cells. CD34 may also play a more selective role in the chemokine-dependent migration of eosinophils and dendritic cell precursors. CD34 plays an important role in cell adhesion, inflammatory cell chemotaxis, cell proliferation and differentiation, and enhancement of inflammatory response[1][2][3][4].

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Rat CD34 at 2 μg/mL can bind Anti-CD34 antibody, the ED50 is 0.0279 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized Rat CD34 at 2 μg/mL can bind Anti-CD34 antibody, the ED50 is 0.0279 μg/mL.

Technical Parameters

  • Species Rat
  • Source HEK293
  • Tag C-hFc
  • Accession

    B1PLB1 (E37-T291)

  • Gene ID
    305081  [NCBI]
  • Molecular Construction
    • N-term
    • CD34 (E37-T291)
      Accession # B1PLB1
    • hFc
    • C-term
  • Protein Length

    Partial

  • Synonyms

    CD34; CD34 Antigen; CD34 Molecule; CD34 Sialomucin; Hematopoietic Progenitor Cell Antigen CD34; Hematopoietic Progenital Cell Antigen CD34

  • AA Sequence

    ENSTQGVFPPVPTNESTEESITSSIPGSTSHYYLIGQDSNKTTPAISETTVNFTATPGTPSGSGTPHTFSQPQTYPTVTLPTTSDSTSTPEVEHSTWNTSLPSVNVSDYSPNNVSSEMISTTEPCAHTSSSVPSIIKGDIKCSGIRGVPFTQGICLELSEASSCEEFKKERGEELMQILCKKEETEADAGTSVCSLNLAQSEVRPECLLMVLANSTELSSKLQLMEKHQSDLRKLGIQSFNKQDLGSHQSYSRKT

  • Molecular Weight

    Approximately 112 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Rat CD34 at 2 μg/mL can bind Anti-CD34 antibody, the ED50 is 0.0279 μg/mL.

  • CD34 Protein, Rat (HEK293, Fc) Product Image
    1/16
  • Experimental Validation Results for CD34 Protein, Rat (HEK293, Fc)
  • Experimental Validation Results for CD34 Protein, Rat (HEK293, Fc)
  • CD34 Protein, Rat (HEK293, Fc) Product Image