CD367/CLEC4A Protein, Rat (HEK293, His)

The CD367/CLEC4A protein is a C-type lectin receptor that prefers to bind mannose and fucose and interacts weakly with GlcNAc in a Ca(2+)-dependent manner. It is critical for immune regulation, undergoing clathrin-dependent endocytosis upon antigen triggering, leading to antigen presentation and cross-priming of CD8+ T cells. CD367/CLEC4A Protein, Rat (HEK293, His) is the recombinant rat-derived CD367/CLEC4A protein, expressed by HEK293 , with N-His labeled tag. The total length of CD367/CLEC4A Protein, Rat (HEK293, His) is 166 a.a., with molecular weight of 25-33 kDa.

CD367/CLEC4A protein functions as a C-type lectin receptor with a preference for binding carbohydrates mannose and fucose, and a weaker interaction with N-acetylglucosamine (GlcNAc) in a Ca(2+)-dependent manner. This receptor plays a crucial role in regulating immune reactivity and, upon antigen triggering, undergoes internalization through clathrin-dependent endocytosis. Consequently, it delivers its antigenic cargo into the antigen presentation pathway, leading to the cross-priming of CD8(+) T cells. Notably, this cross-presentation and cross-priming are augmented by TLR7 and TLR8 agonists, resulting in increased expansion of CD8(+) T cells and heightened production of IFNG and TNF, while reducing levels of IL4, IL5, and IL13. In plasmacytoid dendritic cells, CD367/CLEC4A inhibits TLR9-mediated IFNA and TNF production. Furthermore, it may be involved in the inhibition of B-cell-receptor-mediated calcium mobilization and protein tyrosine phosphorylation via its ITIM motif (immunoreceptor tyrosine-based inhibitory motifs). In the context of microbial infection, CD367/CLEC4A is implicated in the interaction between HIV-1 virus and dendritic cells, enhancing HIV-1 binding and virus infection through an ITIM motif-associated signal transduction pathway involving phosphatases PTPN6 and PTPN11, SYK, Src kinases, and MAP kinases.

Rat

HEK293

N-6*His

Q5YIS1 (Q70-S235)

297584  [NCBI]

QKYSQLLEEKKALTDKTLNDLNCTKNVSLTEDKACSCCLKDWKSFGSYCYFTSTDSKATWDESKEKCSRMGAHLLVIHSQDEQDFINTILNIGTDYFIGLSDHSENQWQWIDQTPYNESVTFWHKGEPNNKEEKCVVINHRDKWGWNDIPCHDRHKSVCQVKKIHS

Approximately 25-33 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.