CD4 Protein, Rhesus Macaque (Biotinylated, HEK293, His)
CD4 Protein forms surface dimers, interacts with LCK and PTK2/FAK1, and binds to P4HB/PDI. It induces CD4-dependent signaling with IL16. CD4 interacts with MHCII alpha and beta chains, increasing TCR affinity. Oligomerization of CD4 is necessary for stable MHCII binding and T cell-APC adhesion. CD4, Rhesus Macaque (Biotinylated, HEK293, His) is the recombinant rhesus macaque-derived CD4 protein, expressed by HEK293, with C-Avi;C-His labeled tag.
- Species: Rhesus Macaque
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
CD4 Protein forms surface dimers, interacts with LCK and PTK2/FAK1, and binds to P4HB/PDI. It induces CD4-dependent signaling with IL16. CD4 interacts with MHCII alpha and beta chains, increasing TCR affinity. Oligomerization of CD4 is necessary for stable MHCII binding and T cell-APC adhesion. CD4, Rhesus Macaque (Biotinylated, HEK293, His) is the recombinant rhesus macaque-derived CD4 protein, expressed by HEK293, with C-Avi;C-His labeled tag.
Background
CD4 protein forms disulfide-linked homodimers at the cell surface and interacts with various proteins, including LCK, PTK2/FAK1, and P4HB/PDI. It also engages with IL16, leading to CD4-dependent signaling in lymphocytes. Furthermore, CD4 interacts with MHCII alpha and beta chains, enhancing the affinity of the T-cell receptor (TCR) for peptide-MHCII. The oligomerization of CD4, particularly via Ig-like C2-type domains 2 and 3, is crucial for stable binding to MHCII and facilitates adhesion between T cells and antigen-presenting cells (APCs).
Technical Parameters
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Species Rhesus Macaque
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Source HEK293
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Tag C-Avi;C-His
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Accession
G7N5T8 (K26-W390)
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Gene ID/
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Molecular Construction
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N-term
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CD4 (K26-W390)
Accession # G7N5T8 -
His-Avi
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C-term
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Protein Length
Partial
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Conjugation
Biotin
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Synonyms
CD4; CD4 Receptor; CD4 Molecule; Surface Glycoprotein Variant; T-Cell Surface Glycoprotein CD4; CD4 Antigen; T-Cell Surface Antigen T4/Leu-3; CD4 Protein; Leu-3; CD4mut; T4; IMD79; CD4 Antigen (P55); OKT4D
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AA Sequence
KKVVLGKKGDTVELTCNASQKKNTQFHWKNSNQIKILGIQGSFLTKGPSKLSDRADSRKSLWDQGCFSMIIKNLKIEDSDTYICEVENKKEEVELLVFGLTANSDTHLLEGQSLTLTLESPPGSSPSVKCRSPGGKNIQGGRTISVPQLERQDSGTWTCTVSQDQKTVEFKIDIVVLAFQKASSTVYKKEGEQVEFSFPLAFTLEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMGKKLPLHLTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQFQENLTCEVWGPTSPKLTLSLKLENKGATVSKQAKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVVPTW
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Predicted Molecular Mass
44 kDa
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Molecular Weight
Approximately 52-58 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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