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CD42a/GP9 Protein, Human (HEK293, Fc)

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CD42a/GP9 protein, forming the GPIb-V-IX complex, acts as the von Willebrand factor (vWF) receptor, facilitating vWF-dependent platelet adhesion to blood vessels—a crucial step in arterial hemostasis. The GP-IX component contributes to the membrane insertion and orientation of GP-Ib, forming a non-covalent linkage with the GP-Ib heterodimer composed of two GP-Ib beta subunits and one GP-Ib alpha subunit. CD42a/GP9 Protein, Human (HEK293, Fc) is the recombinant human-derived CD42a/GP9 protein, expressed by HEK293 , with C-hFc labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • CD42a/GP9 Protein, Human (HEK293, Fc) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

CD42a/GP9 protein, forming the GPIb-V-IX complex, acts as the von Willebrand factor (vWF) receptor, facilitating vWF-dependent platelet adhesion to blood vessels—a crucial step in arterial hemostasis. The GP-IX component contributes to the membrane insertion and orientation of GP-Ib, forming a non-covalent linkage with the GP-Ib heterodimer composed of two GP-Ib beta subunits and one GP-Ib alpha subunit. CD42a/GP9 Protein, Human (HEK293, Fc) is the recombinant human-derived CD42a/GP9 protein, expressed by HEK293 , with C-hFc labeled tag.

Background

The CD42a/GP9 protein, also known as the GPIb-V-IX complex, plays a pivotal role as the von Willebrand factor (vWF) receptor, facilitating vWF-dependent platelet adhesion to blood vessels. This interaction is a crucial initial step in hemostasis, especially in response to injuries in the arterial circulation. GP-IX, within the GPIb-V-IX complex, is involved in the membrane insertion and orientation of GP-Ib. Notably, two GP-Ib beta subunits are disulfide-linked to one GP-Ib alpha subunit, and the GP-IX component forms a non-covalent linkage with the GP-Ib heterodimer.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-hFc
  • Accession

    P14770 (T17-G147)

  • Gene ID
    2815  [NCBI]
  • Molecular Construction
    • N-term
    • GP9 (T17-G147)
      Accession # P14770
    • hFc
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    GP9; Glycoprotein 9; Glycoprotein IX Platelet; Truncated Glycoprotein IX (Platelet); GPIX; Glycoprotein IX (Platelet); Platelet Glycoprotein IX; CD42a Antigen; CD42a; GP-IX

  • AA Sequence

    TKDCPSPCTCRALETMGLWVDCRGHGLTALPALPARTRHLLLANNSLQSVPPGAFDHLPQLQTLDVTQNPWHCDCSLTYLRLWLEDRTPEALLQVRCASPSLAAHGPLGRLTGYQLGSCGWQLQASWVRPG

  • Molecular Weight

    Approximately 48-52 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 250 mM NaCl, pH 8.5, 8% trehalose.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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  • CD42a/GP9 Protein, Human (HEK293, Fc) Product Image