PTPRC/CD45R0 Protein, Human (HEK293, Fc)

Receptor-type tyrosine-protein phosphatase C (PTPRC) is a receptor type PTP and an essential positive regulator of T- and B-cell antigen receptor signaling. PTPRC functions through direct interaction with antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. PTPRC also suppresses JAK kinases as a regulator of cytokine receptor signaling. PTPRC/CD45R0 Protein, Human (HEK293, Fc) is the recombinant human-derived PTPRC/CD45R0 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of PTPRC/CD45R0 Protein, Human (HEK293, Fc) is 416 a.a., with molecular weight of ~122 kDa.

Receptor-type tyrosine-protein phosphatase C (PTPRC) is a member of the protein tyrosine phosphatase (PTP) family, also known as CD45, is a transmembrane glycoprotein. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation.
PTPRC contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP.
PTPRC has been shown to be an essential regulator of T- and B-cell antigen receptor signaling as PTPRC positive regulate T-cell coactivation upon binding to DPP4, recruiting and dephosphorylating SKAP1 and FYN. PTPRC also dephosphorylates LYN, and thereby modulates LYN activity.
PTPRC functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. PTPRC also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling.
PTPRC gene has many alternatively spliced transcripts variants, which encode distinct isoforms^[1][2][3].

Human

HEK293

C-hFc

P08575-4 (M1-K416)

5788  [NCBI]

MTMYLWLKLLAFGFAFLDTEVFVTGQSPTPSPTDAYLNASETTTLSPSGSAviSTTTIATTPSKPTCDEKYANITVDYLYNKETKLFTAKLNVNENVECGNNTCTNNEVHNLTECKNASVSISHNSCTAPDKTLILDVPPGVEKFQLHDCTQVEKADTTICLKWKNIETFTCDTQNITYRFQCGNMIFDNKEIKLENLEPEHEYKCDSEILYNNHKFTNASKIIKTDFGSPGEPQIIFCRSEAAHQGVITWNPPQRSFHNFTLCYIKETEKDCLNLDKNLIKYDLQNLKPYTKYVLSLHAYIIAKVQRNGSAAMCHFTTKSAPPSQVWNMTVSMTSDNSMHVKCRPPRDRNGPHERYHLEVEAGNTLVRNESHKNCDFRVKDLQYSTDYTFKAYFHNGDYPGEPFILHHSTSYNSK

Approximately 122 kDa

Greater than 90% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.